关键词: 牦牛, FSHR基因, 多态性, 繁殖性状, 关联分析

Abstract: To provide a basic information for solving the long-time calving interval in yak through the method of selective breeding,the present study was carried out to analyze the polymorphism of the 5’flank region and exon 1 of the follicle stimulating hormone receptor (FSHR)gene,and to determine their association with reproductive traits.PCR-SSCP assay was used to screen the polymorphisms of FSHR gene in 667 yaks from 4 yak breeds. A least square linear model was used to determine the relationship between the polymorphism of FSHR gene and calving interval.Three fragments of 940 bp in size for yak FSHR gene were amplified and sequenced,including the 5’flank region and exon 1. It had high homology with the corresponding sequences of other mammalian species both at mRNA and amino acid levels.Altogether 9 mutation sites were found and one of them was in the open reading frame with leading to synonymous mutation. The calving intervals among different populations were significantly different (P < 0. 05). No significant difference (P >0.05)existed between calving interval in different genotypes of the FSHR gene,while a trend for shortening calving interval was found in the genotypes of AB, LL,LM,LN and RT.