关键词: 水貂, 卵丘, 泡细胞, 形态结构, 组织化学

Abstract: In this paper，formation of cumulus oophorus in mink(Mustela vison)were studied by light，electron microscope and histochemical techniques．Result showed that，during development of follicle cells，some round m itochondria with few cristae，numerous single rough endoplasmic reticulum cisternae and abundant free ribosomes present in the cytoplasm ．few Golgi complex were seen．Stacks of microfilaments and som e vesicles(1-2um in diameter) that have a thin coat within it’s membrane occur as the follicle cell are unilaminar columnar．Exocytosis could be observed on the plasma membrane of follicle cells，and sometimes zona pellucida material is sandwiched in between these cells adjacent to the oocyte．It indicated that follicle cell might participate in formation of zona pellucida also．As the formation of the autrum ，the granules that contain phospholipids and neutral fat start to occur within follicle cells．During whole development，the follicle cell processes extend through the zona pellucida to anchor on the plasm membrane of the oocyte by means of intercellular attachments．In the later phase of the large antrum follicles，the corona radiate cells extend and make a obvious radial shape，opposing against the outer aspect of the zona pelucida．The extension appear in other cumulus cell in varying degrees too．Sheaves of microfilaments alternate with rough endoplasmic reticulum cistenae，and they run parallel to major axis within the extended cytoplasm of the corona radiate cells．It indicated that microfilaments play a great role in the extention of follicle cells．The extention of cumulus cells make whole cumulus oophorus turn into porous and filled with hyaluronic acid．It would be advantageous to sperm’s penetration and getting into oocyte afterwards．

Key words: Mink, Cumlus oophorus, Follicle cells, Morphological structure