关键词: 大熊猫, RAPD 分析, 分类学, Southern 杂交

Abstract: We employed PCR and Southern-blot hybridization to analyze a shared 1.3 kb DNA fragment among the RAPD products from giant panda, lesser panda, malayan bear and raccoon genomic DNA. The results showed that the 1.3kb fragment from giant panda may be a repetitive sequence, which contained several binding sites of the random primer AB1-08 (GTCCACACGG). The southern-blot hybridization result with the 1.3kb fragment from giant panda as probe showed that the counterpart DNA fragment from malayan bear has strong hybridization signal while those from lesser panda and raccoon have no signal that could be detected. It suggested that giant panda might has closer relation ship with bears than that with lesser panda and raccoon and should be classified into the Ursidae family with malayan bear. Our research result provide another evidence for the solution of the riddle of giant panda taxonomy position on molecular biology level.

Key words: Giant panda (Ailuropoda melanoleuca), RAPD analysis, Taxanomy, Southern-blot hybridization