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林麝和马麝随机扩增多态DNA的研究

包惠芳,徐宏发,陆厚基   

  1. 华东师范大学生物系,上海
  • 出版日期:2006-06-23 发布日期:2008-07-07

STUDIES ON FOREST MUSK DEER AND ALPINE MUSK DEER USING RANDOM AMPLIED POLYMORPHIC DNA (RAPD)

BAO Huifang,XU Hongfa,LU Houji   

  • Online:2006-06-23 Published:2008-07-07

摘要: 用随机扩增多态 DNA(RAPD)技术对饲养的林麝和马麝进行分子遗传标记研究。在选用的42 种随机引物中, 有25种引物产生了清晰稳定的条带, 单个引物获得标记数在1~14 之间,平均每个个体获得168个RAPD标记 , 其中林麝、马麝特异性标记各5个,个体特异性标记有3个, 这些标记可用来鉴定种或个体。平均遗传距离在林麝种内为0.27±0.023, 马麝为0.105±0.013, 种间为0.241±0.02 , 种间差异显著大于种内差异。分析表明饲养马麝种内遗传多样性低, 为增强饲养马麝的生存力, 最好从不同种群中引入种麝进行繁殖。

关键词: 林麝, 马麝, 随机扩增多态 DNA (RAPD)

Abstract: Randomly Amplified Polymorphic DNA(RAPD) was employed to screen the genetic markers in Forest musk deer and Alpine musk deer. About 168 RAPD markers were generated in each individual with 42 primers,5 special markers were found in Forest musk deer and Alpine musk deer respectively, and also 3 individual special markers were generated,these can be used to identify species or individuals. The average genetic distances in Forest musk deer and Alpine musk deer and between them were 0.124±0. 023、0.105±0.013、0.241±0.02,respectively. The results indicated that the difference detected by proportion of shared fragments between the species was obviously larger than those within a species.The population of Alpine musk deer need new individuals from different populations to avoid inbreeding depression.

Key words: forest musk deer(Moschus moschiferus), Alpine musk deer(Moschus chrysogaster), RAPD