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棕色田鼠小肠肌间神经丛NOS 的组织化学观察

刘梅,徐金会,安书成,邰发道   

  1. 陕西师范大学生命科学学院
  • 出版日期:2006-05-24 发布日期:2008-07-07

HISTOCHEMICAL STUff OF NITRIC OXIDE SYNTHASE IN THE MYENTERIC PLEXUS OF MICROTUS MANDARINUS SMALL INTESTINE

LIU Mei,XU Jinhui,AN shucheng,TAT Fadao   

  • Online:2006-05-24 Published:2008-07-07

摘要: 用NDP- 黄递酶组织化学法研究了NOS在棕色田鼠(Microtus mandarinus)小肠肌间神经丛的分布,同样的方法对大白鼠进行了实验比较。结果发现,棕色田鼠小肠肌间神经丛NOS阳性神经元形态各异,大小悬殊数倍;在神经节和阳性神经纤维中可见阳性神经元呈“串珠”状和“U”型排列。10 倍镜下记数100 个视野,观察到阳性神经元平均密度为42.8个/mm2,每个视野内平均有神经节12.4个,每个神经节内平均有NOS 阳性神经元8.5个,占其神经节内神经元总数的27.2%。棕色田鼠为野生草食性动物,其NOS 在小肠肌间神经丛的分布与其他杂食性啮齿类有相同之处,但也存在着种属差异。

关键词: 一氧化氮合成酶, 棕色田鼠, 小肠, 肌间神经丛, 组织化学

Abstract: The intramural neurons containing NOS in the small intestine of the Microtus mandarinus were studied by using NADPH-diaphorase histochemical method. The results showed: (1) The shapes and sizes of the positive neurons were different. In the ganglion and nerve strand, there existed a bread-like and"U" -shaped structure. (2) 12.4 ganglions are in every field and 8.5 neurons in every ganglion. (3) The proportion of NOS neurons is 27.2%. (4) The density of positive neurons are about 42.8 per square millimetre. Comparing with the experimental animals such as the rats the similarity and the difference were both existed.

Key words: Microtus mandarinus, Nitric oxide synthetase (NOS), Small intestine, Myenteric plexus, Histochemistry