关键词: 马鹿, 卵母细胞, 体外培养, 体外受精, 超微结构

Abstract: The aim was to study the role of ultrastructural changes of wapiti oocytes before and after cultivation and fertilization in vitro. The oocytes were collected by aspiration and deep cut methods from deer that had been injected with FSH, for superovulation. The oocytes were selected from different developmental stages and their ultrastructure was observed under the Transmission Electron Microscope. Cultivation and fertilization in M199 was carried out at 38.5℃ under a humidified atmosphere of 5% CO₂ in the air. The results revealed that before the oocytes were cultivated in vitro,the cumulus cells surrounded them tightly;long thin microvilli were on their surface and deep into the zona pellucida. There were a lot of organelles in the area of the cortex and the centre of the cells. After the oocytes were cultivated in vitro,the link between cumulus cells and oocytes became loose,the microvilli lying on the oocytes surface shortened and thickened,the first polar body had no nucleus,the cortical granules were distributed in layers in the cortex area,and the organelles were distributed evenly in the cytoplasm. When the oocytes were fertilized in vitro,the microvilli on their surface became erect, the second polar body had a nucleus,and in the cytoplasm of the oocytes there were abundant organelles which were mainly distributed in the centre of the cells.

Key words: In vitro cultivation, In vitro fertilization, Ooctye, Ultrastructure, Wapiti (Cervus elaphus)