关键词: 荧光定量RT-PCR, TaqMan 探针, 犬瘟热病毒, M 基因

Abstract: To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains. The reactive conditions were optimized to improve the sensitivity and specificity of the method. The specificity and sensitivity of the method were high. Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR, the common RT-PCR and electron microscopy. The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy. This suggests that the method may be used in clinical diagnosis,epizootic study and aboratory research.

Key words: CDV, M gene, RT-PCR, TaqMan-based probe