Abstract: In order to determine the cause of death of a Sichuan golden monkey (Rhinopithecus roxellanao),Necropsies were carried out according to a standard protocol. Tissue samples were fixed in 10% neutral buffered formalin solution and embedded in paraffin. The 4 μm sections were deparaffinized,stained and processed routinely for pathohistology analysis. Most samples were examined for detecting bacteriums by inoculating into the broth medium and viruses by Vero,PK-15,BHK-21 cells culture,morphology and reverse transcription-polymerase chain reaction (RT-PCR). Then the KM mice regression test were carried out by the intraperitoneal injection with different isolated strains. The results showed that Cardiac pericardium,epicardium,and endocardium appeared bleeding point and ecchymosis;cardiac muscle appeared white necrotic focus;lungs were swollen and congestive. Cardiac muscle fiberes were abound with inflammatory cell infiltrate;parts of them were broke,degenerated. The heart appeared typical myocarditis changes in which the blood capillary of alveolar wall highly stretched and congested. The viruses (Picornaviridae-like morphous)and bacteria (Bacillus coli) were isolated from the samples of golden monkey. The KM mice regression test showed that the bacteria had no pathogenicity on mice.
However,the viruses isolated could make mice infected,and it also showed the similar pathologic symptoms with the Sichuan golden monkey. The RT-PCR performed on an extract of cardiac muscle tissue revealed a DNA sequence specific for Coxsackievirus B. Our results indicated that the myocarditis caused by the Coxsackievirus B resulted in Sichuan golden monkey's death.

Key words: Etiopathogenesis, Myocarditis, Pathology, Sichuan golden monkey(Rhinopithecus roxellanae)

摘要： 对临床送检的急性死亡川金丝猴进行解剖,观察各组织脏器的大体病理变化并对其进行中性甲醛固定、石蜡包埋切片和病理组织学分析;无菌操作进行肉汤法分离培养细菌和细胞培养法分离病毒,通过形态学、动物回归试验和RT-PCR 方法对分离的病原进行培养、鉴定。结果,眼观心肌呈局灶性坏死,镜下心肌纤维断裂、崩解,肌间有大量炎性细胞浸润,呈典型的病毒性心肌炎变化;从肺脏中分离获得大肠杆菌;电镜负染可见心肌组织匀浆液和心包积液以及其Vero 细胞培养物中均有直径在20 ～ 25 nm,形似小RNA 病毒的粒子存在;RTPCR检测结果说明所分离病毒为柯萨奇B 型病毒;动物回归试验证实,分离的大肠杆菌对昆明种小鼠无致病性,而分离的病毒对小鼠有致病性,且病理组织学变化与金丝猴相似。根据临床症状、病理剖检和病理组织学变化特征,结合病原分离结果综合分析,推测该金丝猴死亡的原因可能与该病毒感染有关。

关键词: 川金丝猴, 心肌炎, 病理学, 病因