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Structural and functional identification of Toll-like receptor genes in wild boar

YANG Xiuqin,LI Haitao,XU Yang,ZHAI Chunyuan,MA Jianzhang,LIU Di   

  1. College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China
  • Online:2011-12-06 Published:2011-03-02

野猪Toll 样受体基因的结构和功能鉴定

杨秀芹, 李海涛,徐扬, 翟春媛, 马建章, 刘娣   

  1. 东北农业大学动物科技学院,哈尔滨 150030

Abstract:

Toll-like receptors (TLRs),which play an important role in the immune responses, are candidate genes for resistance to a large number of diseases in animals. In order to provide more useful information for analyzing the structure and function of TLRs, this study was designed to clone TLR4/5 genes in Wild boar and to characterize their tissue expression patterns using RT - PCR. The complete coding sequences of Wild boar TLR4/ 5 genes were obtained, and each predicted polypeptide contained important structural domains including signal peptide, LRRs and TIR. Wild boar TLR4 /5 genes were expressed ubiquitously in all tissues tested. The results will provide molecular biological evidence further revealing the structure and function of TLR4/5.

Key words: Cloning, Tissue expression, TLR4/ 5, Wild boar

关键词: 野猪, TLR4 /5, 克隆, 组织表达