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Expression of interleukin-6 in gastrointestinal tract of streptococcal pneu monia rhesus macaques

LUO Qihui,CHEN Lian,ZHANG Yao,CHEN Zhengli,CHENG Anchun,ZENG Wen   

  • Online:2012-02-29 Published:2012-02-20

感染肺炎链球菌猕猴消化系统白细胞介素6 的表达观察

罗启慧, 陈恋,章瑶, 陈正礼,程安春, 曾文   

  1. 四川农业大学动物医学院

Abstract: We examined the expression of interleukin-6 in the gastrointestinal tract,liver and esophagus in rhesus macaques infected with Streptococcus pneumoniae spontaneously.The pathogenesis of streptococcus pneumonia and features of spontaneous Streptococcus pneumoniae infection are discussed.Using common H & E staining,general histological and pathological changes were detected,and the changes of interleukin-6 expression in liver,stomach,jejunum,caecum and esophagus were analyzed by immunohistochemistry and in situ hybridization. All of the organs inspected showed inflammatory reactions. Hepatic tissue and enteradans were necrotic。The liver,jejunum and caecum showed obvious hemorrhage and hyperemia.Many inflammatory cells infiltrated the gastrointestinal tract. Compared with normal control groups,infected groups had significantly higher expression of interleukin-6 (P < 0.05). The total areas of positive cells in the infected groups were also significantly higher than those in normal monkeys (P <0. 05). Most of the positive cells were concentrated in the mucous layer,while in the liver,were mainly distributed around the blood vessels. Glandular cells,hepatocytes, endotheliocytes,uncuticular epitheliums and lymphocytes all showed positive reactions.As an inflammatory factor,interleukin-6 may play a role in Streptococcus pneumoniae infections and may participate in the clearance of Streptococcus pneumoniae.

Key words: Immunohistochemistry, Interleukin-6, In situ hybridization, Mucous layer, Streptococcus pneumoniae

摘要: 检测猕猴自发性感染肺炎链球菌后,白细胞介素6 在胃肠道以及肝脏、食管的表达变化,探讨肺炎链球菌的发病机制以及自发性肺炎链球菌性肺炎的病理特点。采用常规H. E.染色观察消化系统病理组织学变化,采用免疫组织化学和原位杂交方法检测白细胞介素6 在肝脏、食管、空肠、盲肠和胃组织的表达变化。各组织表现出典型的炎症病变,肝组织和肠腺均可见大面积坏死,在空肠、盲肠和肝脏中有明显的广泛性出血和充血现象;肠道有大量炎性细胞浸润。和健康组比较,白细胞介素6 在感染组的胃肠道以及肝脏、食管中的表达均呈现升高趋势,感染组的IL-6 阳性细胞总面积比正常组有显著性升高
(P < 0.05)。在胃肠道以及食管的表达主要集中在粘膜层,在肝脏相对集中分布于血管周围。阳性细胞包括了部分腺体细胞、肝细胞、内皮细胞、未角化
上皮细胞以及淋巴细胞。白细胞介素6 作为一种炎症细胞因子在肺炎链球菌感染中发挥了一定的作用,可能参与了肺炎的病理过程并对机体清除肺炎链球菌有一定促进作用。

关键词: 肺炎链球菌, 白细胞介素6, 粘膜层, 免疫组化, 原位杂交