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Mitochondrial DNA D-loop polymorphism among four populations of Reed voles (Microtus fortis)

XIE Jianyun,FENG Jie,SHAO Weijuan,BAI Xiong,HU Jianhua,GAO Cheng   

  • Online:2007-12-11 Published:2008-07-07

东方田鼠四个种群线粒体D-loop 区遗传多态性分析

谢建云, 冯洁, 邵伟娟, 柏熊, 胡建华,高诚   

  1. 上海实验动物研究中心,上海实验动物监督检验站,上海

Abstract: Four populations of Reed voles (Microtus fortis) were used to check genetic polymorphisms of the mitochondrial DNA (mtDNA)D-loop by means of RFLP analysis.The PCR fragments of this region were digested with 15 restriction endonucleases: 10 with no variation and the other five including Eco T22I, Eco RV, Hap II, Hind III and Xba I, indicating polymorphisms among different individuals.Ten haplotypes were eventually recovered among sampled 40 individuals.The
genetic divergence among these haplotypes ranged respectively from 0. 0014 to 0.0168. All vole samples clustered into two distinct branches:Voles from Dongting Lake and some voles from Qingtongxia comprised one branch,and voles from Heilongjiang and the rests from Qingtongxia comprised the other branch. These findings suggested that the mtDNA D-loop region in this species provides informative variations for population research and phylogeography in the future.

Key words: Genetic polymorphism, Mitochondrial DNA (mtDNA), RFLP, Reed vole (Microtus fortis)

关键词: 东方田鼠, 线粒体DNA (mtDNA), RFLP, 遗传多态性