Abstract: The multisubunit NADH ubiquinone oxidoreductase (complex I)is the first enzyme complex in the electron transport chain of mitochondria. The iron-sulfur protein (IP)fraction is made up of 7 subunits,including NDUFS6 (NADH-ubiquinone oxidoreductase Fe-S protein 6). In order to understand the giant panda’s NDUFS6 gene and coding of protein using RT-PCR combined with in silico cloning,we isolated and sequenced the cDNA encoding NADH-ubiquinone oxidoreductase iron-sulfur protein 6 of the giant panda (Ailuropoda melanoleuca). The deduced protein sequence showed that the protein is composed of 124 amino acids and the estimated molecular weight of the NDUFS6 protein is 13.632 kDa with an isoelectric point (PI)of 9.51 Alignment analysis reveals that the deduced protein sequence shares 85.6 % ,82.4% ,76.6 % and 76.1 % homology with that of Homo sapiens,Bos taurus,Rattus norvegicus and Mus musculus,respectively. In particular,there are 2 Protein kinase C phosphorylation sites (₁₄ SGK₁₆ and ₅₁ SRK₅₃ )in the NDUFS6 protein of the giant panda.

Key words: Cloning Giant panda RT-PCR NDUFS6 Sequences analysis

关键词: 大熊猫 RT-PCR NDUFS6 克隆 序列分析