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A new method for detecting exon 2 alleles of DRB genes in sika deer

LI Bo,XU Yanchun,MA Jianzhang   

  1. College of Wildlife Resoures,Northeast Forestry University,Harbin 150040,China
  • Online:2010-03-02 Published:2010-03-02

梅花鹿DRB 第二外显子等位基因鉴定的一种新方法

李波, 徐艳春, 马建章   

  1. 东北林业大学野生动物资源学院,哈尔滨 150040

Abstract: A new method was found which integrated Motif specific-PCR,SSCP and direct sequencing to identify the exon 2 of alleles of DRB genes in sika deer. Using this method,the detected 15 sequences which included no artificial sequences from mutation or recombination in the process all met the criterion to distinguish a new allele. The criterion is that there have to be identical sequences identified from at least two different individuals by PCR and direct sequencing. Compared with PCR-SSCP-cloning sequencing previously,this method can efficiently remove artificial sequences. Combined with the above criterion,this method provided a new approach for investigating DRB gene polymorphism of sika deer and other deer species.

Key words: Alleles, DRB, Motif specific-PCR, Polymorphism, Sika deer (Cervus nippon)

关键词: 梅花鹿, DRB 基因, 等位基因, 多态性, 基序特异性PCR