ACTA THERIOLOGICA SINICA ›› 2018, Vol. 38 ›› Issue (6): 597-607.DOI: 10.16829/j.slxb.150225

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The development of in vitro models and their applications in cetacean research

YU Xueying, YANG Feng, ZHU Jingmin, MING Yao, LI Ding, LIU Wenhua,WANG Jingzhen#br#   

  1. (1 Qinzhou University, Guangxi Key Laboratory of Marine Disaster in the Beibu Gulf, Qinzhou 535011,China)
    (2 Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou 515063,China)
      (3 College of Environment Science and Engineering, Guilin University of Technology, Guilin 541006,China)
  • Online:2018-11-30 Published:2018-11-26

体外模型的发展及其在鲸豚研究中的应用

于学颖 杨峰 朱静敏 明瑶 李鼎 刘文华 王京真   

  1. (1 钦州学院,广西北部湾海洋灾害研究重点实验室,钦州 535011)
    (2 广东省海洋生物技术重点实验室,汕头 515063)
    (3 桂林理工大学环境科学与工程学院,桂林 541006)
  • 通讯作者: 王京真 E-mail: wangjingzhen-1@163.com

Abstract:

As an alternative and complementary in vitro models, “cell line” provides a new platform for life science research. To a certain extent, it breaks through the limitations of ethics, law, animal welfare and animal protection in scientific research and reveals the biological effects and regulatory mechanisms of complex living organisms from a cellular and molecular perspective. Especially for endangered animals, the establishment of cell lines combined with cryopreservation technology can not only preserve the genetic germplasm of endangered animals with biological expression activity, but also provide a new platform for in vitro conservation research (such as animal toxicology experiments), which is of great significance to animal protection. Cetacean cell culture has so far proven to be a multifunctional tool for cytogenetics, virology and toxicology studies. However, from the perspective of species, tissue sources and cell types, long-term stable continuous cell lines are still relatively few. How to optimize cell culture conditions and use the cell lines to reveal more information about cetacean life mechanism is still a challenge. This paper reviews the development of in vitro models and its application in the study of cetaceans, with a view to promoting the innovation of this technology in the study of cetacean conservation.

Key words: Cetaceans, In vitro model, Cell line, Toxicology

摘要:

体外补充替代模型“细胞系”为生命科学研究提供了新的平台,在一定程度上突破了科学研究中伦理、法律、动物福利和动物保护等的限制,从细胞和分子视角更深层次地揭示复杂生命体的生物效应和
调控机制。尤其对于濒危动物,细胞系的建立与超低温冷冻技术相结合,既可以保存濒危动物具有生物表达活性的遗传种质,又可以提供体外保育研究的新平台(如动物毒理学实验),对动物保护意义
重大。目前细胞培养体系已作为多功能平台被应用于鲸豚类细胞遗传学、病毒学和毒理学的相关研究中,但从物种和组织来源以及细胞类型来看,能长期稳定传代的鲸豚类细胞系仍较单一。优化细胞培
养条件,运用鲸豚类体外细胞揭示更多的生命机制问题,仍是当前鲸豚类体外细胞模型研究所面临的挑战。本文对动物体外模型及其在鲸豚研究中的应用进行了概述,以期推动该技术在鲸豚保育研究中
的创新和发展。

关键词: 鲸豚类, 体外模型, 细胞系, 毒理学