%0 Journal Article %A CAI Qingxiu %A LIN Liu %A LUO Shujin %A PAN Wenjing %A ZHANG Li %T Screening microsatellite DNA markers and genetic variation analysis of wild
Asian elephant population in Mengyang Nature Reserve

%D 2008 %R %J ACTA THERIOLOGICA SINICA %P 126-134 %V 28 %N 2 %X To get the appropriate microsatellite markers,we first screened 31 loci originally developed in the African elephant and five in the Asian elephant (Elephas maximua) using one muscle sample and then test the locus polymorphism and analyze genetic variation by the total 191 faecal elephant samples collected in Mengyang Nature Reserve through 4 - 8 repeat PCR and genotyping. The results showed 14 loci from the 36 can amplify in the target muscle DNA sample and verified to be microsatellite after sequencing. Nine microsatellite loci were optimized for faecal DNA amplification in the targeted Asian elephant population. For Mengyang population,we obtained amplified product from 185 of the total 191 samples (96.9%) and 55 unique genotypes. 3 loci deviated from Hardy-Weinberg equilibrium and at least 8 loci lacked linkage. The average number of alleles is 3.78 ±1. 72, the average expected and observed heterozygosity and PIC were 0.32 ± 0. 06, 0. 36 ±0.02 and 0.28, respectively,which indicated that these loci were suitable to genetic researches for Asian elephant. The relatively low heterozygosity (0.32 ±0.06) and allele frequencies compared with other populations showed that the genetic variation was low and genetic structure was potentially skewed in the Mengyang population. %U http://www.mammal.cn/EN/abstract/article_2183.shtml