%0 Journal Article %A HUA Yuping %A LIU Lanlan %A ZENG Xiangwei %T Genetic variations of haemagglutinin protein gene of canine distemper virus isolated from breeding fox and raccoon dog %D 2008 %R %J ACTA THERIOLOGICA SINICA %P 217-220 %V 28 %N 2 %X Haemagglutinin (H) protein genes of six CDV strains isolated from fox and raccoon dog between 2004 and 2006 were amplified by RT-PCR, and then were cloned and sequenced. Their sequences were also compared with those of 34 CDV reference strains in Genbank. The results show that the ORF of H genes of six strains are all composed of 1 824 nucleotides, encoding a polypeptide of 607 amino acid residues. Deletion and insertion were not found in the H genes of the six isolates. Phylogenetic tree based on nucleotide sequences of H genes from six isolates and 34 reference strains show all these strains are classified into six majority genetic lineages, designated Asia - 1, Asia - 2, America - 1, America - 2, Europe, Arctic. HL isolate has high homology with Italy strain 194/97 and Danmark strain Green, and belongs to Arctic genetic lineage. The other five isolates have high homology with Japan strains HAMA,UENO,Tanu96 and China strains TN, GP, and belong to Asia -1 genetic lineage, this shows a certain extent geographical relativity. The six isolates have lower homology with vaccins strains Onderstepoor and Convac, it shows that virus variation may be one of important reasons for prevalence of CD in vaccinated animals. %U http://www.mammal.cn/EN/abstract/article_2197.shtml