%0 Journal Article %A CHEN Guihua %A DU Yurong %A GUO Songchang %A GUO Xinyi %A LIN Gonghua %A PANG Bo %A XIE Ling %A ZHANG Xuze %T CDS cloning and sequence analysis of neuronal nitric oxide synthase (nNOS)from plateau zokor (Myospalax baileyi) %D 2014 %R %J ACTA THERIOLOGICA SINICA %P 17-27 %V 34 %N 1 %X Enduring hypoxia stress related to high altitude and subterranean burrows,the plateau zokor (Myospalax baileyi) has high efficiency in oxygen utilization which makes it adapt very well to the hypoxic plateau environment. Neuronal nitric oxide synthase (nNOS),one of three isoforms of nitric oxide synthase,plays an important role in the adapation to hypoxia. In
this study,the coding sequence of nNOS was cloned by RT-PCR from the brain of plateau zokor. The size of this gene is 4 290 bp in length,and encodes a protein composed of 1 429 aa. The nNOS gene of the plateau zokor was 87% to 90% homologous to human,dog,rabbit,mouse and rat. We identified typical nNOS domains,such as PDZ, oxidase, reductase domain and CaM binding sites within nNOSs of Plateau zokor. The molecular phylogenetic trees based on ML and Bayesian method showed that the plateau zokor clustered with mouse and rat,which was consistent with traditional phylogenetic relationship inferred from morphological and molecular markers. Three positive selected sites of Plateau zokor (332 T,1200 G and 1334 P)were identified by the PAML analysis,although statistically insignificant. Our results lay a foundation for further exploring the expression pattern,function and regulatory mechanism of nNOS in Plateau zokor acclimatization to hypoxia. %U http://www.mammal.cn/EN/abstract/article_2953.shtml