ZHOU Yucan, HU Xiaomei
摘要： 两种基因型不同的亲本之间的杂交试验是遗传学家和育种工作者熟悉的手段。杂种优势通常在高度近交系间的杂交子代中是常见的,也是育种学家赖以培养新品种的依据。 实验小白鼠(Mus musculus albino)是从野生小家鼠(Mus musculus)培育而来的。Klein(1975)和Moriwaki等(1979)认为现在广泛应用的实验小白鼠很可能来源于中国和日本的小家鼠。但是这2种近亲系小鼠的杂交试验至今未见报道。
Abstract: To study penetic characteristics of the ariots proteins in the mise (Mus musculus),the hybridization of wild morse (grey fur) and latoratory mouEe(albino)was tested.lie;esvlts of hybridization and linkage of hemog-lobin with fur coolr locus are reported in this paper.The mating group,incltding 4 pairs of wild rouse(female)with laboratory mouse (male), has not genertated for 8 months.Cn the contrary,the other tra-ting group(4 pairs)of wild mouse (male)with latoratory mouse(ferrale)gene-rated offspring after about two months. The sum of F1 is 70 for 7 foetus in the latter group, and all individuals were of grey fur. It is evident that the grey fur is a dominant character and the white fur is a recessive character.Among 14 pairs of sib mating (F1), there are 7 pairs to generated offspring,the other 7 pairs are sterile,For fur,colour of F2 of 55 individuals the great malority are of grey fur,some are of white fur,only a few are of yellow or black fur. We regard all colour individuals as none albinos, then compared the sum of colour individuals with the those white ones(albinos). The ratio is 3.23:1,being agreed with Mendel's laws. There are also grey, white,yellow and black fur in the offspring of backcross. When the sum of colour individuals compare with the white ones,the ratio is 0.87:1,being in accordance with Mendel's laws,too.The hemoglobins of P,F1 and F2 were analysed by gel electrophoresis.It is found that in F1or F2, all hemoglobin patterns of grey fur are similar to those of wild mouse(male parant),in F2 all Hb patterns of white fur are not only similar to,but also identical with those of laboratory mouse(female parant).For example,when Hb of female parant is "single type",the Hb of F2(white fur) is also "single type"; when Hb of female parant is "diffuse type",the Hb of F2(white fur)is"diffuse type",too.It is obvious that linkage exists in the hereditary chaacters between herropobin and fur colour.Among Hb of hybrid,the new component of Hb vas not yet found. However,due to diffeience of beterozyosity of F1,the two molymorghic types of Hb are appeared.It is just a reflection of sex difference in F1. Similarly,due to difference of segregation of F2 the Hb polymorphism also occurred.For grey,yellow and black fur mice,the difference of Hb is not yet observed. Comparisicn on all patterns of Hb ccnfirrred that slow migrating components are probably important to decide what the fur colovr cf mouse will be.
周虞灿, 胡晓梅. 野生小家鼠与实验小白鼠杂交世代的血红蛋白电泳分析[J]. .
ZHOU Yucan, HU Xiaomei. ELECTROPHO RETIC ANALYSIS OF HEMOGLOBIN ON THE HYBRID GENERATIONS OF WILD MOUSE AND LABORATORY MOUSE[J]. .