关键词: 高原鼠兔, 大鼠, 低氧, 肝代谢, 转氨酶, 溶酶体酶

Abstract: Effects of chronic hypoxia on liver of Ochotona curzoniae and rats have been carried out by simulated altitude (5000m,Po₂ 84mmHg, O₂ 11%) in hypobaric presure chamber for 15 days, and compared with those at 2300 m of altitude (120mmHg of Po₂, O₂ 16%).In fourth filial generation of rats removed from the lowland to Xining (2300 m of altitude)the levels of content of glycogen,lipid and protein mar- kedly decreased from 753 ± 34.9, 869±23.2 and 1113 ± 11.3 to 384±25.8,606 ±23.8 and 814 ± 8.1 mg/whole liver respectively(P<0.001)during exposuring to 5000 m altitude for 15 days, while the activities of lysosomal enzymes, acid phcsphatase and aryl sulpbatase, enharced significantly (P<0.001), whereas SGPT and SGOT kept unchanged. The biochemical changes were supported by hepatic morphological evidence that hypoxia led to cloudy swelling and ballooning of cells and parenchymal cell necrosis. However none of changes had been observed in these indexes mentioned above in native mammal pika (O.curzoniae) at the same experimental conditions.The data, therefore, suggested that obvious different mechanism of adaptation to hypoxia chronically in hepatic metabolisms and enzymes related to hepatic function and damage of liver cells necrosis existed between rats and O. curzoniae. The perfect mechanisms uniformed structure and functions of acclimatization to altitude in liver of O. curzoniae were formed by long term of evolution at cellular and molecular level. The model of O. curzoniae would serve us for searching the principle of adaptation based on molecular biology to hypoxia. Much attention must be paid in terms of effects of hy-poxic hypoxia either acutely or chronically upon liver clinically.

Key words: Pika, Ochotona curzoniae, Rat, Hypoxia, Liver metabolism, Aminotransferase, Lysosomal enzymes