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鹿科动物血清蛋白质的SDS—聚丙烯酰胺凝胶电泳分析

王宗仁1, 贾凤兰2, Massoud Mohomdi3   

  1. 1. 中国科学院动物研究所;
    2. 中国预防医学科学院病毒研究所;
    3. Wadley分子医学研究所 美国
  • 出版日期:2011-11-23 发布日期:2011-11-22

AN ELECTROPHORETIC ANALYSIS OF THE SERUM PROTEIN IN CERVIDAE-SDS-PAGE

WANG Zongren1, JIA Fenglan2, Massoud Mohomdi3   

  1. 1. Institute of Zoology, Academia Sinica;
    2. Institute of Virology, Chinese Academy of Preventive Medicine;
    3. Wadley Institutes oj Molecular Medicine, U.S.A.
  • Online:2011-11-23 Published:2011-11-22

摘要: 本文对鹿科动物(黑鹿、白唇鹿、黇鹿、白黇鹿、麋鹿、东北马鹿、甘肃马鹿、中亚马鹿、日本梅花鹿、东北梅花鹿、狍)的2个亚科、4个属、7个种进行了血清蛋白质的SDS—聚丙烯酰胺凝胶电泳测定。结果表明,鹿的血清蛋白在该种电泳方法中,共可分辨出分子量在1.24—9.5×104范围内的53个蛋白区带。其中最少的是白唇鹿,黇鹿为24条,最多的是日本梅花鹿为35条。

关键词: 鹿, 血清, 蛋白质, 电泳

Abstract: The paper reported that SDS-PAGE of serum protein of Cervidae. These deer were Cervus unicolor, Cervus albirostris,Dama dama,Elaphurus davidianus, Cervus elaphus xanthopygus, Cervus elaphus macneilli, Cervus elaphus bactrianus,Cervus nippon nippon, Cervus nippon hortulorum and Capreolus capreolus.They belong to 2 subfamilies,4 genus, 7 species.The results obtained are as follows:There were 53 bands of the serum protein of SDS-PAGE on these deer. The molecu- lar weight were different to each other and were between 1.24-9.5 × 104 dorton. The serum in MERIONES MERIDIANUS and D, dama were separated into 24 components, the number of bands were the lowest. But the serum in C. nippon nippon there were 35 separaated components, the number of bands were the highest. The results of comparison of the bands in different subfamilies different genus, different species and between subspecies showed that:1. In evolution of deer, the changes in serum protein were more strenuous than in Karyotype, but their main trend of the change in evolution was same. The more in lower taxonomical unit,the more there were similar components of serum protein.2. The smallest different of serum protein between genus were Cervus and Dama, secondly, were Cervus and Elaphurus, the largest were between Elaphurus and Dama.3. According to the comparison of the serum protein, the genealogical tree in evolution was from C. unicolor to MERIONES MERIDIANUS to C. elaphus to C. nippon. This was very similar to the order in evolution of karyotypes of deer.4. There were very biggest difference between serum protein of cow and of deer in electrophoretic comparison of the serum protein.

Key words: Deer, Serum, Protein, SDS-PAGE