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高原鼠兔乳酸脱氢酶同工酶对低氧环境的应答

刘国富, 温得启, 韩思梗, 胡晓梅   

  1. 中国科学院西北高原生物研究所
  • 出版日期:2011-11-23 发布日期:2011-11-22

LACTATE DEHYDROGENASE ISOENZYME RESPONDS TO HYPOXIA IN PIKA

LIU Guofu, WEN Deqi, HAN Sigeng, HU Xiaomei   

  1. Northwest Plateau Institute of Biology, Academia Sinica
  • Online:2011-11-23 Published:2011-11-22

摘要: 用聚丙酰胺凝胶薄层电泳和紫外光谱法,研究与分析高原鼠兔(Ochotona curzoniae)在天然及模拟低氧条件下,心脏、肝脏、肾脏及骨骼肌4种组织的乳酸脱氢酶(LDH)同工酶的酶谱和其酶活力的变化。

关键词: 高原鼠兔, 乳酸脱氢酶, 同工酶, 高原适应, 生物化学

Abstract: The lactate dehydrogenase(LDH) isoenzymes from heart, liver, kidney and skeletal muscle in the pika (Ochotona curzcniae) were analysed by means of the polyacrylamide gel electrophoresis and the ultraviolet enzymatic determination at a simulated altitude of 5,000m for 15 days. The following conclusions were obtained:1. Pikas were exposed to a simulated altitude of 5,000 m for 15 days. Their changes of M-subunit ratio in crude extract of the heart, liver and kidney were no significance in the electrophoretic isoenzyme patterns of LDH, as compare with those of alt. 2300m.2. The exposure of the pika to simulated altitude oxygen conditions for 15 days resulted in a significant alteration in skeletal muscle LDH isoenzyme patterns. The proportion of M-subunit of LDH increased approximately by 14% from 2,300m to 5,000m altitude.3. Continuous exposure of the pika to a simulated altitude of 5,000 m for 15 days seems to be no effect on the LDH activities of heart, liver, kidney and skeletal muscle (P>0.5).

Key words: Pika, Ochotona curzoniae, Lactate dehydrogenase, LDH, Isoenzyme, Altitude acclimatization, Biochemistry