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江豚MDH与ADH同工酶电泳研究

陆佩洪, 余多慰, 马玉银   

  1. 南京师范大学生物系
  • 出版日期:2011-11-23 发布日期:2011-11-22

STUDIES ON MDH AND ADH ISOENZYMES IN NEOPHOCAENA PHOCAENOIDES WITH ELECTROPHORETIC TECHNIQUE

LU Peihong, YU Duowei, MA Yuyin   

  1. Department of Biology, Nanjing Normal University
  • Online:2011-11-23 Published:2011-11-22

摘要: 用琼脂糖凝胶载玻片电泳方法测定了江豚(Neophocaena phocaenoides)体内几种组织的MDH和ADH同工酶。测定结果表明,各组织MDH同工酶酶谱基本相同,ADH同工酶酶谱呈现一定的组织特异性,且各同工酶活性可因底物或pH的改变而变化。

关键词: 苹果酸脱氢酶MDH, 醇脱氢酶ADH, 琼脂糖凝胶电泳, 江豚

Abstract: Using electrophoretic method, a study of MDH and ADH isozymes in 3 individuals of Neophocaena phocaenoides (♂ 2,♀ 1 ) has been made. The electrophoreto grams of MDH isozymes in different tissues are similar. 1 m-MDH and 1-2 s-MDH bands are detected from all examined samples. The average content of s-MDH is more than that of m-MDH in main organs.The electrophoretograms of ADH in Neophocaena phocaenoides is as follows: 1 band to cathode and 0-4 bands to anode have been found, when the substrate is used with n-butyl alcohol or isopropyl alcohol. The activity of ADH is detected in all examined tissues, and the ADH isozymes are somewhat special from one tissue to the others.The electrophoretograms vary with different substrate and pH.

Key words: Malic dehydrogenase, Alcohol dehydrogenase, Electrophoresis in agarosegel, Neophocaena phocaenoides