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华南兔组织乳酸脱氢酶同工酶的研究

周虞灿, 姜科声   

  1. 浙江师范大学生物系
  • 出版日期:2011-11-23 发布日期:2011-11-22

STUDIES ON LACTATE DEHYDROGENASE ISOZYME OF TISSUES FROM SOUTH CHINA HARE, LEPUS SINENSIS

ZHOU Yucan, JIANG Kesheng   

  1. Department of Biology, Zhejiang Normal University, Jinhua
  • Online:2011-11-23 Published:2011-11-22

摘要: 本文以聚丙烯酰胺凝胶电泳法分析华南兔(Lepus sinensis sinensis)11种组织乳酸脱氢酶同工酶的分布待征。分析结果:骨骼肌和肝组织5条同工酶带俱全;脑、肺、卵巢和盲肠等组织各含有4条谱带(LDH-1,-2,-3和-4);胃和肾组织含有3条谱带(LDH-1,-2,和-3);眼晶状体和睾丸组织也含有3条谱带,但前者是LDH-3,-4和-5,后者是LDH-1,-2和-5;谱带最少的是心肌组织,只有2条(LDH-1和-2)。此外,还对各组织中的亚基活性分布及电泳图谱特征进行了分析。

关键词: 华南兔, 组织, 乳酸脱氢酶同工酶变种, 凝胶电泳法

Abstract: This paper deals with the tissue specificity of lactate dehydrogenase isozyme in South China hare.Eleven tissue LDH isozymes have been analysed by means of poly-acrylamide gel electrophoresis. It has been found from results that there are five zones in tissues of muscle and liver;four zones(LDH-1,-2,-3 and -4)in the brain,lung,ovary and caecum;three in the stomach,kidney,eye and testis; and two(LDH-land LDH-2)in the heart. However, Certain general correlation have been noted, M subunits have the preponderance in tissues of muscle and lens while H subunits in tissues of heart, brain, lung,stomach, kidney, ovaey and caecum, and both M and H subunits, come close to equality in tissues of liver.The electrophoretic variants of LDH isozymes in tissues of South China hare are found.The sub-bands appeared within six LDH isozyme zones of three tissues(stomeah,kidney and ovary).

Key words: South China hare, Lepus sinensis, Tissue, Lactate dehydrogenase isozyme variant, Gel ectrophoresis