关键词: 大林姬鼠, α-酯酶, β-酯酶, 苹果酸脱氢酶, 同工酶, 组织特异性

Abstract: The thin—layer isoelectric focusing electrophoresis with polyacrylamide gel method wereused to analyse the α-esterase(α-est),β-esterase(β-est)and Malate dehydrogcnase(M DH ) isozymes in the five kinds of tissues of Apodemus peninsulae．The sampies covered heart，liver，spleen，kidney and muscle．The results show that all the three kinds of isozyme have obvious tissue specificity．There are clear different enzyme activities in the five tissues．The Esterase activity in liver is higher than in other four tissues．The zymograms in the different issues have distinct differences as well. Spleen
is different from other tissues，its β—Est only existing in region and band B．In the same tissue，the α-Est and β-Est are different too．Normally，the activity of α-Est is
higher than ofβ-Eat．The MDH of liver has an evident region AB when it is stained in alkaline solution,but spleen has none．The MDH activities of heart and muscle are
a little higher than of other tissues．

Key words: (Apodemus peninsulae, α-esterase, β-esterase, Malate dehydrogenase, isozyme, Tissue specificity