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达乌尔鼠兔和高原鼠兔肝脏线粒体DNA的限制性内切酶图谱的比较研究

王东江, 曾伟强   

  1. 中国科学院遗传研究所
  • 出版日期:2006-07-25 发布日期:2007-09-13

C0MPARATIVE STUDY 0F RESTRICTⅣE MAPS 0F MIT0CH0NDRIAL DNAS FROM LIVERS 0FOCHOTONA DAURICA AND 0.CURZONIAE

WANG DongJiang, ZENG Weiqiang   

  • Online:2006-07-25 Published:2007-09-13

摘要: 达岛尔鼠兔肝细胞线粒体DNA(mtDNA),经限制性内切酶HindI、HindII,EcoRI和BamHI酶切后分别产生5、4、3、2个片段。通过琼脂糖凝肢电泳对这些片段进行测定,并画出其酶切图谱。高原鼠兔肝细胞mtDNA经限制性内切酶EcoR I和BamH 1酶切后,分别产生4、2个片段,对其片段的分子量也进行了测定。测定结果,达乌尔鼠兔肝细胞mtDNA的分子量为10.25MD(兆道尔顿).大小为16.25kbp(千碱基对);高原鼠兔肝细胞mtDNA的分子量为9.31MD,大小为l5.066kbp。并对两种鼠免的限制性内切酶片段进行了比较讨论。

关键词: 达乌尔鼠兔, 高原鼠兔, 线粒体DNA, 限制性内切酶

Abstract: mtDNA from Ochotona daurica liver was cleared by HindⅡ,HindⅢ ,EcoRI and BemHI into 5,4,3,and 2 fragments.The sizes and orders of these fragments in the respective genomes were determined with data obtained from agarose gel electrophoresis.mtDNA from O.curzouiae liver was cleaved by EcoRI and BaraHI into 4 and 2 fragments.The sizes were determined by the same way as in 0.deurica.The molecular weight of 0.daurica mtDNA wes found to be l0.25 MD and about 16.59 kbp; the molecular weight of O.curzoniae mtDNA was 9.31 M D and about 15.066 kbp;The differences of restrictive fragments between ratDNA from 0.daurica and 0.curzoniae were discussed.

Key words: Mouse-hare, Plateau pika, mtDNA, Restriction enzyme