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### 三种鼠组织超氧化物歧化酶和乳酸脱氢酶同工酶的比较研究

• 出版日期:2006-07-26 发布日期:2007-09-13

### COMPARATIVE STUDY OF SUPEROXIDE DISM UTASE AND LACTATE DEHYDROGENASE ISOZYMES IN THE TISSUES FROM THREE KINDS 0F MURIDS

ZHOU Yucan, SHAO Lingxiang, HE Xinxia

• Online:2006-07-26 Published:2007-09-13

Abstract: Superoxide dismutase (SOD) and lactate dehydrogenase (LDH ) isozymes in the tissues of three wild rats have been compared by simultaneous development of their activity staining on a single slab ge1．The migratory velocity of LDH zones of A .agrarius is slower than that of R.norvegicus and R.norvegicus，so that two different zymograms appeared：the former is a close-together pattern while the latter is a loose-pattern．It is suggested that the chemical structure of their subunits be different．The zone of LDH—X (C4) is found in the testis tissue of the oestrus male rat．This zone is located on the negative electrode side of LDH -5 in A.agrarius，but it is located between LDH—4 and LDH一5 in R．norvegicus．
the migratory velocity of SOD isozyme zones in the tissues of A.agrarius is also different from that of R.flavipectus and R .norvegicus．The former is faster，so in the same tissue the distance from LDH-1 to S0D main band is farther apart。the 1atter is slower, so in the same tissue the distance from LDH-l t0 S0D main band is much closer．The test of heat-resisting proved that the ability of endurance of subunit B of LDH is much higher than that of subun it A，but lower than that of S0D．The test of cyanide inhibition proved that the SOD mentioned above belongs to Cu／Zn-SOD ．In the tissues of all infant rat the number of S0D zones and their distributions are obviouely different rom those of an adult rat．Thisf act suggests that the change of SOD molecular structure occur along with in dividual development from infant to adult for R. norvegicus