• • 上一篇    下一篇

腹腔注射对氯苯丙氨酸对达乌尔黄鼠冬眠的影响

黄钦恒, 蔡益鹏, 张崇理, 王红   

  1. 北京大学生物系
  • 出版日期:2006-07-29 发布日期:2007-09-13

THE ROLE OF BRAIN SEROTONERGIC SYSTEM IN HIBERNATION OF GROUND SQUIRREL

HUANG Qinheng, CAI Yipeng, ZHANG Chongli, WANG Hong   

  • Online:2006-07-29 Published:2007-09-13

摘要: 本工作采用腹腔注射5-羟色胺合成抑制剂对氯苯丙氨酸降低脑内5-HT含量的方法,观察了脑内5-HT系统在达乌尔黄鼠自然冬眠和脑室注射6-羟多巴胺促进入眠效应中的作用。结果表明;在自然情况下,达乌尔黄鼠入眠后脑内5-HT含量增加,腹腔注射对氯苯丙氨酸后动物入眠诱导期明显延长;在脑室注射6-羟多巴胺人为地降低脑内NE系统活动的条件下,脑内5-HT含量即使处于较低水平,动物也能很快入眠,但入眠时脑内5-HT含量需要恢复到一定的基础水平。提示脑内NE系统功能活动的降低或/和5-HT系统功能活动加强同是触发动物入眠的重要因素。周期性入眠和觉醒可能依赖于脑内这两个系统功能活动的平衡。

关键词: 达乌尔黄鼠, 冬眠, 对氯苯丙氨酸, 5一羟色胺, 去甲肾上腺素

Abstract: In this work the role of brain serotonergic system in natural hibernation and hibernation induced by i.c.v.injection of 6-hydroxydopamine were studied in ground squirrel (Citellus dauricus)after intraperitonal administration of para-chloro phenylalanine(pACA ). a specifiedepletor of brain serotonin. The results showed: (1) The contentof 5-HT in whole brain, after pCPA injection was depleted to 42% of control valueat 2nd day, and reached to the lowest at 4th day, about 19%of control, then gradually recovered with time, arrived at 84%of control value at 16th day. (2) In thenatural hibernating ground squirrels, the induction period for hibernation were 26± 6days。and 5-HT content in brain increased when compared with the euthermic animals,while the induction period for hibernation in ground squirrels pretreated with pCPAwere 35±5 days, significantly longer than that of natural hibernating animals. (3)The ground squirrels treated with i.p. pCPA +i.c.v.6-oHDA were able to enter into hibernation when 5-HT content in brain has not recovered to the control level.howeverthe induction period for “ hibernation (15±5 days) was longer than that of animalstreated with i.P.saline+i.c.v.6-OHDA, indicating that a basal 5-HT level was stillnecessary for hibernating under the condition of i.c.v. injection of 6-OHDA.
These results sugested that both the decrease of NE system activity or/and theincrease of 5-HTsystem in brain are the important controlling factors in triggeringthe onset of hibernation.

Key words: Ground squirrel (Citellus dauricus), Hibernation, Para——Chloropheny-lalanine, Serotonin, Norepinephrine