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刺猬冬眠过程中褐色脂肪和非颤抖性产热研究

孙金生, 曾缙祥   

  1. 天津师范大学生物系
  • 出版日期:2006-07-29 发布日期:2008-01-05

STUDIES ON THE VARIATIONS OF BROWN ADIPOSE TISSUE AND NONSHIVERING THERM OGENESIS IN A HIBERNATOR (ERINACEUS EUR0PAETUS)

SUN Jinsheng, ZENG Jinxiang   

  • Online:2006-07-29 Published:2008-01-05

摘要: 本文对刺捐(Erinaceus europaeus)在冬眠过程中褐色脂肪(BAT)的相对重量、超微结构和非颤抖性产热(NST)的变化进行了测定.探讨了BAT产热在冬眠刺猬醒眠过程中的作用。结果表明,夏季(7月份)刺猬的BAT相对重量最低,为0.23%。秋季迅速增加,至入眠前达到最高,为0.46%。冬眠期中缓慢减少,冬眠后显著下降(0.32% )。这与醒眠时需要快速产热密切相关。冬眠前和冬眠中刺猬的BAT脂滴和线粒体嵴数量明显高于冬眠后。表明冬眠刺猬BAT具备快建产热能力。在冬眠室温为5℃时的静止代谢率最低,为0.064毫升氧/克/小时,活动期,即在0℃至下临界沮度范围内,其静止代谢率与环境温度呈负相关,并具线性回归。冬眠期的NE-NSTmax明显低于活动期,而相对增加耗氧量显著高于活动期,证明NE-NST是冬眠刺猬酲眠时的主要热源。

关键词: 刺猬, 褐色脂肪, 非颤抖性产热, 静止代谢率

Abstract: The variations of nonshivering thermogenesis (NST ) and brown adipose tissue (BAT ) in weight and uhrastrueture were studied in the hedgehogs (Erinaceus europaeus),so as to deal with the function of BAT during arousaI from hibernation. The results showed that,the relative weight of BAT in hedgehogs was the lowest (0.23% )in summer(July),andinereased rapidlyin autumn,the highest value of relative weight was observed in the prehibernation (November);no variations were observed in the hibernating hedgehogs,but decreased rapidly during arousal from hibernation (0.32 %).The variation of BAT were related to the heat production during arousa1.The numbers and sites of fat droplets and mitachondria cristaes of BAT in the prehibernating and hibernating hedgehogs were higher than that in the posthibernating hedgehogs,and show the hibe rnating hedgehogs could be able to produce heat rapidly during arousal from hibernatoin.
The results also showed that the thermoneutral zone was the ranges 25℃ to 27℃ and the lowest RMR (mlO2/g/h)was 2.577士0.654 in the awaking hedgehogs,over the Ta.ranges 0℃ to the low artical tem perature,a significantly negative correlation and a regression was observed between the Ta.and the RMR:The lowest RMR was 0.064 at th e Ta.of 5℃ in the ibernating hedgehogs,and the Ta.also affected RMR.
The NE— NST expressed in two forms:the maxmium metabolic response to NE (NSTmax),and the absolute increase in oxygen consumption ( (NSTmax—RMR)/RMR ).NSTmax in the awaking hedgehogs was higher than that in the hibermating hedgehogs,and show the body temperature of hibernating hedgehogs inhibited the metabolic rate;The absolute increase in oxygen consumption in the awaking hedgehogs was lower than that in the hibernating hedgehogs,and show the NE- NST was the main heat source during arousal from hibe rnation.

Key words: Hedgehog (Erinaceus europaeus), Brown adipose tissue (BAT), Nonshivering th~ mogenesis (NST), Resty metabolic rate (RMR).