金属诱导条件下刺猬各组织器官金属硫蛋白含量的比较分析

金属诱导条件下刺猬各组织器官金属硫蛋白含量的比较分析

李令嫒，马宏宝，吕迎春，茹炳根

北京大学生命科学学院生物化学及分子生物学系，北京

出版日期:2006-07-29 发布日期:2008-07-07

ANALYSIS 0N METALL0THIONEIN C0NTENT 0F 0RGANS AND TISSUES OF HEDGEH0G UNDER THE C0NDIT10N 0F METAL INDUCEMENT

LI Lingyuan， MA Honghao，LU Yinghun，RU Binggen

Online:2006-07-29 Published:2008-07-07

摘要/Abstract

摘要： 本文采用Ag-Hb(血红蛋白)饱和法、Cd—Hb饱和法、Cu-Hb饱和法及酵联吸附免疫法定量分析刺猬各组织器官中金属硫蛋白含量。结果表明，肌肉注射三种金属盐均有明显的诱导金属硫蛋白合成的作用，诱导强度为Ca²⁺ >Zn²⁺>Cu²⁺，诱导后金属琉蛋白含量分布规律一致，即肝>肾>睾丸>脾>心、肺、胃、膀胱、胆汁、大腑、小肠、肌肉、血液，其中镉诱导肝的金属硫蛋白含量每克湿组织约1.84mg，为肾的1倍、睾丸的3倍、脾的5倍、其余组织器官的7-5倍。对肌内注射金属盐的抗性为Zn²+>Cd²⁺>Cu²⁺ ，每公斤体重注射zn²⁺80mg、Cd²⁺ 40mg，Cu²⁺ 20mg时，出现明显的中毒反应,并开始死亡.金属l硫蛋白对四种金属离子的结合能力为Ag⁺>Cu⁺ >Cd⁺>zn⁺ 。

关键词: 刺猾, 组织器官, 金属硫蛋白

Abstract: Muscle injecting metal salts CdCL₂，CuSO₄ and ZnSO₄ into hedgehog bodies,the comparative resistance to metals for hedgehogs were Zn²⁺ > Cd²⁺ > CU ²⁺，and the serious toxicity of metals on hedgehogs appeared when the injecting amount reached Zn²⁺ ·8Omg,Cd²⁺ 4O mg Or Cu²⁺ ·2O mg／body·kg．According to metal-hemoglobin saturation (AgCd,Cu)assay and enzyme-linked immunosorbent assay,Cd ²⁺．Zn²⁺ and Cu²⁺ all had obvious，effect of inducing the synthesis of MT，and the inducing potency by metals was Cd²⁺ > Zn²⁺ > Cu²⁺ ．In the three kinds of inducing conditions，the comparative MT concentrations in different organ tissues of hedgehog were the same order as liver>kidney> testis> spleen> heart，lung，stomach，bladder，bile，intestine，muscle，and blood．In Cd²⁺ inducing condition，the MT content in liver was 1.84mg／g．wet tissue,about two times as in kidney，three times as in testis，five times aS in spleen，and 7-15 times as in other organs．The potent order of hedgehog MT binding with metals were Ag²⁺ > Cu²⁺ > Cd²⁺> Zn²⁺．

Key words: Hedgehog (Erinacaus eurapaeus), Organ and tissue, Metallothionein

李令嫒，马宏宝，吕迎春，茹炳根. 金属诱导条件下刺猬各组织器官金属硫蛋白含量的比较分析[J]. .

LI Lingyuan， MA Honghao，LU Yinghun，RU Binggen. ANALYSIS 0N METALL0THIONEIN C0NTENT 0F 0RGANS AND TISSUES OF HEDGEH0G UNDER THE C0NDIT10N 0F METAL INDUCEMENT[J]. .