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大熊猫DNA指纹分析材料的初步研究

方胜国, 丁朝武, 冯文和, 张安居, 李光汉, 李绍昌, 余健秋, 李学兵   

  1. 四川师范大学分子生物学研究所,成都
  • 出版日期:2006-08-02 发布日期:2008-07-07

A PRELIMINARY STUDY ON THE MATERIAL RESOURCE OF DNA IN THE DNA FINGERPRINTING ANALYSIS OF GIANT PANDAS

FANG Shengguo, Ding Zhaowu, FENG Wenhe, ZHANG Anju, LI Guanghan, LI Sahochang, YU Jianqiu, LI Xuebing   

  • Online:2006-08-02 Published:2008-07-07

摘要: 以圈养大熊猫4个家系,13只个体的31份粪便,尿液以及甲醛固定的睾丸组织等做材料,辅以血液被毛进行DNA指纹分析.结果表明,同一个体的血液、被毛、粪便、尿液、以及甲醛固定的睾丸组织材料的DNA指纹图谱完全一致.使用两种寡核苷酸探针重复验证圈养大熊猫5胎6仔的父子关系,效果稳定.

关键词: 大熊猫, DNA指纹

Abstract: DNA were isolated from formalin-fixed testes, excrements, urine, blood and hairs of 13 giant pandas in 4 families in Chengdu Zoo and Chengdu Research Base of Giant Panda Breeding.Using the method of DNA fingerprinting we made an analysis of the DNA finger-prints to the 4 families, and obtained the following results: 1. The DNA fingerprints of blood, hairs, urine, fornalin-fixed testes and excrements of the same giant panda are identical. It indicates that all the materials above are the resource of DNA in the research works of DNA fingerprinting analysis of giant panda. 2. Both of the oligonucleotide probes of JH12.6 provided by Jinhe Applied Biotechnoligy Institute and LZF-1made by Molecular Biology Institute of Sichuan Normal University can form the hybrid molecules with the simple repetitive DNA of genome of giant pandas, and aa of the hybrid bands of any fillal generation farrowed down by monogamy and polyandry come from their parents and the blood relationship of 4 families have been determined again bu us. 3.Because the DNA can be obtained from the urine, excrementd, formalin-fixed tissues, haris and blood and there is an identical result in the DNA fingerprinting analysis, and therefore the achievement ushered in a vast vistas in the material resouce of DNA fingerprinting analysis of ferocious animals and the animals of being in imminent danger.

Key words: Giant panda, DNA fingerprinting