六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析

六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析

金晓玲，何新霞，周虞灿，吴林富，干常春

浙江师范大学生物系，金华

出版日期:2006-08-02 发布日期:2008-07-07

ANALYSES ON VARIATION OF MDH ， HEMOGLOBIN AND SOME PLASMA PROTEINS FOR SIX MURIDS

JIN Xiaoling , HE Xinxia , ZHOU Yucan , WU Linfu , GAN Changchun

Online:2006-08-02 Published:2008-07-07

摘要/Abstract

摘要： 本文分析了家鼠属（Rattus）4种鼠和姬鼠属（Apodemus）2种鼠的苹果酸脱氢酶、血浆中B₁区和B₂区蛋白质、血浆请蛋白、前清蛋白以及红细胞中血红蛋白的变异。结果表明，MDH在进化上是比较保守的，其中MDH_M和MDH_S2带在各鼠间位于同一泳动线上，仅MDH_S1带的泳动速度出现属间和种间的微小差异；血浆中B₁区和B₂区的蛋白质带、清蛋白带以及前清蛋白带各鼠间出现明显的差异，表现为黑线姬鼠和社鼠在B₁区出现2种多态型区带，褐家鼠在B₂区也有2种多态型带；各鼠间的血红蛋白变异比较明显，主要表现在区带数和主成分的泳动度显著不同，而且社鼠的Hb 出现3中多态型，黄毛鼠出现2种多态型，其与各鼠的Hb 皆为单态性。上述16项生化特性按相似和相异进行配对比较，初步表明黄毛鼠与褐家鼠亲缘上比较相近，白腹巨鼠要比其他3种鼠亲缘上更接近于黑线姬鼠，而社鼠与白腹巨鼠之间进化分歧相对的较大。

关键词: 生化分类, 鼠科动物, 血浆蛋白质, 血红蛋白, 苹果酸脱氢酶

Abstract: The MDH isozymes , hemoglobins and some plasma proteins of 4 species of the Rattus genus and 2species of the Apodemus genus were analysed by means of the vertical slab gel elect rophoresis . The results obtained showed that the MDH isozymes were rather conservative in evolueion . The MDH_M and MDH_S2 zones among all species analysed were respectively located to the same migratory line , but migratory speed of the MDH_S1 zones were different between the species of the two genus . The variations of migratory speed on the zones of albumin , pre –allbumin , the proteins in B₁and B₂ regions among the species were found . There were 2 polymorphic types in the B₁ region of A . agrarius and R . niviventer , respectively . There were also 2 polymorphic types in the B₂ region of R . norvegicus .It was also found to the variations of the migratory speed and the number of Hb zones among all species analysed . There were 2Hb polymorphic types for R. rattoid and 3 for R. niviventer . The phonetic analysis with 16 biochemical characters by the pair-group method showed that R . norvegicus was closed to R . rattoides in ally. The R . edwardsi was closer to A . agrarius than the res 3 species of the Rattus in ally . There were relatively marked divergence between R. edwardsi and R . niviventer in evolution .

Key words: Biochemical systematics, Murid, Plasma protein, Malate dehydrigenase(MDH), Hemoglobin(Hb)

金晓玲，何新霞，周虞灿，吴林富，干常春. 六种鼠苹果酸脱氢酶、血浆蛋白质和血红蛋白的变异分析[J]. .

JIN Xiaoling , HE Xinxia , ZHOU Yucan , WU Linfu , GAN Changchun. ANALYSES ON VARIATION OF MDH ， HEMOGLOBIN AND SOME PLASMA PROTEINS FOR SIX MURIDS[J]. .

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