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六种鼠组织中过氧化物酶和酯酶变异的比较

何新霞, 金晓玲, 周虞灿,郑祥建, 沈晖   

  1. 浙江师范大学生物系
  • 出版日期:2006-06-29 发布日期:2008-07-07

STUD IESON VARIATION OF PEROXIDASE AND ESTERASE FOR SIXMURIDS

HE Xinxia, JIN Xiaoling, ZHOU Yucan, ZHENG Xiangjian, SHEN Hui   

  • Online:2006-06-29 Published:2008-07-07

摘要: 本文研究姬鼠属(A podemus) 2种鼠和鼠属(Rattus) 4种鼠的心组织过氧化物酶和肝组织酯酶同工酶等位基因的变异。结果表明, 6种鼠心组织过氧化物酶皆为4条区带, 其中社鼠和白腹巨鼠的酶带泳动速度略快于褐家鼠和黄毛鼠的酶带; 鼠属4种鼠的酶带间距大致相同, 而姬鼠属的酶带间距略大于鼠属, 这种谱型的区别就所分析的6种鼠而言, 可以认为是该同工酶表现的属间区别。肝组织酯酶区带较多, 其酶带数和泳动速度的差异具有明显的种间特征; 泳动最快的酶带在姬鼠中是2条, 在鼠属中是1条, 可以认为是属间特征的区别。从9项生化指标的异同作动物配对比较, 表明褐家鼠与黄毛鼠亲缘上较近, 社鼠与白腹巨鼠亲缘上也较近, 而且白腹巨鼠在进化上可能要比其余3种鼠属动物更接近于姬鼠属。

关键词: 生化分类, 过氧化物酶, 酯酶, 鼠科动物

Abstract: The allelic variations of peroxidase in the tissues of heart and esterase isozymes in the tissues of liver for two species of the genus Apodemus and four species of the genus Rattus were analysed to studies on biochemical systematics of the Rattus group. the results obtained showed that there are four zones of peroxidase in the tissues of heart for each species and their migratory velocities were faster for the R. niviventer and R. edwardsi species than for the R. rattoides and R. norvegicus species. The distances between the each two zones were farther for the genus Apodemus than for the genus Rattus. This feature may bet reated as the difference between the two genera. The number of the esterase zone and their mobilities were obviously different from the species to the species.The difference of the esterase isozymes between the two generalie in that there is one zone with the fastest movemen t in the genus Rattus and two zones with the fastest movement in the genus Apodemus. The phenetic analysis with the nine biochemical characters seems in ally the R. ra ttoides is closed to the R. norvegicus, and the R. niviventer is closed to the R. edwardsi,When they are compared without group species, the R. edwardsi is nearer to the species of Apodemus than the other three species in the genus Rattus.

Key words: Biochemical systematics, Murid, Peroxidase, Esterase, Isozyme