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中国和韩国黑线姬鼠两亚种的线粒体DNA限制性片段分析

王金星 高兴善   

  1. 山东大学生物系
  • 出版日期:2006-04-07 发布日期:2008-07-07

ANALYSIS OF MITOCHONDRIAL DNA RESTRICTION FRAGMENT PATTERNS IN TWO SUBSPECIES OF STRIPED FIELD M ICE APODEMUS AGRAR IUS FROM NORTHERN CHINA AND KOREA

WANG Jinxing HUNG Sun Koh   

  • Online:2006-04-07 Published:2008-07-07

摘要: 本文利用印迹杂交技术对中国华北地区黑线姬鼠Apodemus agrarius pallidior和韩国黑线姬鼠A . agrarius coreae共107号标本的线粒体DNA (mitochondrialDNA , mtDNA ) , 通过8种限制性内切酶的消化, 进行了限制性片段的分析。 共检出35种限制性片段和12 种单倍体类型。12种单倍型在平均离散度为1.01%时聚合为两个亚群: 一个亚群为黑线姬鼠华北亚种, 由采自中国4个不同地区的51号标本的4种单倍型所组成; 另一个亚群为黑线姬鼠朝鲜亚种, 由采自韩国4个不同地区的56号标本的8种单倍型所组成。  黑线姬鼠华北亚种和朝鲜亚种在mtDNA 表型上表现出一定差异, 这在分子水平上确立了两亚种的分类地位。为了进一步澄清黑线姬鼠种下分类的混乱, 很有必要对中国其他地区的标本进行该项研究。

关键词: 分类学, 线粒体DNA, 黑线姬鼠, 中国华北地区, 韩国

Abstract: One hundred and seven samples of two subspecies of striped field mice, Apodemus agrarius pallidior from Northern China and A . agrarius coreae from Korea, were used for the analysis of mitochondrialDNA (mtDNA ) fragment patterns resulted from the digestion with eight restriction enzymes by blot hybridization technique.   All 35 fragments and 12 mtDNA haplotypes were recognized. The 12 hap lotypes were grouped into two major subgroups with the mean divergence of 1.01%. One subgroup with four haplotypes consisted of 51 samples from four localities in Northern China, which is A . agrarius pallidior, and the other subgroup with eight haplotypes is composed of 56 samples from four localities in Korea, A . agrarius coreae.  Subspecies coreae appeared to be some what diverged in their mtDNA geno types from subspecies pallidior, and taxonomic status of these two subspecies is confirmed in this molecular study. It becomes necessary to carry out further researches with samples of striped field mice from other localities to clarify subspecies classification of A . agrarius.

Key words: Taxonomy, Mitochondrial DNA (mtDNA ), Apodemus agrarius, Northern China, Korea