野生大家鼠和实验大白鼠血红蛋白和乳酸脱氢酶同工酶的比较研究

野生大家鼠和实验大白鼠血红蛋白和乳酸脱氢酶同工酶的比较研究

黄贵萍　黄钦皂　郭光红廖若男刘　菲

贵阳医学院

出版日期:2006-04-07 发布日期:2008-07-07

COMPARATIVE STUDIES ON THE HEMOGLOBIN AND LACTATE DEHYDROGENASE ISOZYMES FROM TWO KINDS OF WILD AND LABORATORY RATS

HUANG Guiping　　HUANG Qinzao　GUO Guanghong LIAO Ruonan　LIU Fei

Online:2006-04-07 Published:2008-07-07

摘要/Abstract

摘要： 本文对野生大家鼠(Rattus norvegicus) 和Wistar品系实验大白鼠的血红蛋白(Hb) 及组织的乳酸脱氢酶(LDH) 同工酶进行了电泳分析, 结果表明, 两种鼠均表现了Hb的多态性,其中Hb I 型都含有8个成分, 各成分的泳动率相同。两种鼠的心脏LDH 同工酶都含有LDH1,LDH2, LDH3和LDH5, 而且各谱带泳动率相近似, 其余组织LDH 谱带泳动率有明显的差异,表现了组织和种(或品系) 的特异性。从电泳图谱上发现Wistar 大鼠LDH5 (A或M亚基纯合体) 出现亚带, 而LDH1 (B或H 亚基纯合体) 则未发现, 可确定变异是由于A或M亚基位点发生突变, 出现等位基因而导致。对褐家鼠的LDH4出现亚带也进行了讨论。

关键词: 野生大家鼠, 实验大白鼠, 血红蛋白, LDH同工酶, 凝胶电泳

Abstract: The hemoglob in sand lactate dehydrogenase (LDH) isozymes in wild rat ( Rattus norvegicus ) and wistarst rain of laboratory rat have been analysed by means of polyacrylamide gel disc electrophoresis. All animals adult. The results are as bellows: 1. The biochemical polymorphism of the hemoglobin was found in two kinds of wild and laboratory rats, Rattus norvegicus has 3, 6, 7 and 8 components; wistar strain rat has only 8 components. Hemoglobin typeⅠof both rats possessed eight zones and their migration rates (PlateⅠ and F ig.1) and scanning curves (F ig.2) were similar. 2. Each tissue LDH isozyme in Rattus norvegicus and wistar strain rat was found to have a characteristic distribution of these isozymes in the pattern, but the isozyme bands in heart have appeared LDH1, LDH2, LDH3 and LDH5 and migration rates were similar, in other tissue the numder and themobility of LDH iszoymes were different (P lateⅠ ). 3. The electrophoretic variants of serum and tissue LDH isozyme from wistar strain are described, since sub-band have appeared with in LDH5 and LDH4, it indicates that LDH-A (M ) locus occurs in mutant alleles.

Key words: Wild rat ( Rattus norvegicus ), Laboratory rat (Wistar strain ), Hemoglobin, LDH, Polyacrylamide gel disc electrophoresis

黄贵萍　黄钦皂　郭光红廖若男刘　菲. 野生大家鼠和实验大白鼠血红蛋白和乳酸脱氢酶同工酶的比较研究[J]. .

HUANG Guiping　　HUANG Qinzao　GUO Guanghong LIAO Ruonan　LIU Fei. COMPARATIVE STUDIES ON THE HEMOGLOBIN AND LACTATE DEHYDROGENASE ISOZYMES FROM TWO KINDS OF WILD AND LABORATORY RATS[J]. .

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