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低氧对高原鼠兔肝细胞内质网和心肌肌浆网Ca2+ 泵的影响

吴雁, 杜继曾, 尤治秉   

  1. 中国科学院西北高原生物研究所
  • 出版日期:2006-06-29 发布日期:2008-07-07

THE EFFECTS OF STIMULATED HYPOXIA ON LIVER ENDOPLASMIC RETICULUM AND HEART SARCOPLASMIC RETICULUM Ca2+ -PUMP OF OCHOTONA CURZONIAE

WU Yan, DU Jizeng, YOU Zhibing   

  • Online:2006-06-29 Published:2008-07-07

摘要: 模拟高原低氧条件研究高原鼠兔肝细胞内质网(Endoplasmic reticulum, ER) 和心肌肌浆网(Sarcoplasmic reticulum, SR) 钙泵功能变化。实验设对照组(海拔2300m) 和两个低氧实验组(模拟海拔5000m 和7000m)。24 h 急性低氧时, 海拔5000m 组高原鼠兔ER 的Ca2+ 泵活性无变化, 海拔700m 组高原鼠兔ER Ca2+ 泵活性下降29.02%。7 d 亚急性低氧时高原鼠兔SR 的Ca2+ 泵活性无显著变化。高原鼠兔ER 的Ca2+ 泵活性在海拔5000 m 组和7000 m 组分别升高32.50% 和33.33%。25 d 慢性低氧时高原鼠兔ER, SR 的Ca2+ 泵活性均无显著变化.表明: 急性低氧对Ca2+ 泵功能有抑制作用, 低氧7 d 后抑制缓解, 至25 d 低氧时趋于恢复。 

关键词: 低氧, Ca2+ 泵, 高原鼠兔, 内质网, 肌浆网

Abstract: The effects of stimulated hypoxia on Ca2+ -pump activity of the liver endoplasmic reticulum (ER ) and heart scarcoplasmic reticulum (SR ) of Ochotona curzoniae (pika) were studied. Inthis experiment Ochotona curzoniae were exposed to simulated altitudes of 5 000m and 7 000m in hypobaric pressure chamber for 24 hours, 7 days and 25 days.The effects of different altitude and different duration of hypoxia onCa2+ -pump activity are not same. After exposed to 7000m hypoxia for 24 hours, Ca2+ -pump activity of pika ER was inhibited 29.02% (P < 0.1). After 7 days of hypoxia Ca2+ -pump activity of ER and SR increase 32.59% (P < 0.05) and 33.33% (P < 0.01) at altitude of 5 000 m and 7 000 m. During chronic hypoxia of 25 days, theCa2+ -pump activity of were unchanged.

Key words: Hypoxia, Ca2+ -pump, Ochotona curzoniae, Sarcoplasmic reticulum, Endoplasmic reticulum