• •    下一篇

邛崃和相岭山系小熊猫种群的遗传结构

魏辅文  饶刚  李明  冯祚建   

  1. 中国科学院动物研究所
  • 出版日期:2005-07-12 发布日期:2008-07-07

POPULATION GENETIC STRUCTURE OF RED PANDA ( AILURUS FULGENS) IN QIONGLAI AND XIANGLING MOUNTAINS

WEI Fuwen RAO Gang LI Ming FENG Zuojian   

  • Online:2005-07-12 Published:2008-07-07

摘要: 测定了来自小熊猫主要分布的两大山系——相岭和邛崃的23 只小熊猫样品的mtDNA控制区5’端569 bp 序列, 共发现23 个变异位点, 定义了8 种单元型, 其中邛崃山系5 种,相岭山系3 种。分析结果表明, 两山系均表现出较低的核苷酸多态性; 单元型多态性差异明显, 其中邛崃山系高于相岭山系。分子变异分析表明, 两山系的小熊猫种群间具有较高的基因流( Nm = 2.95) , 两种群遗传分化不明显(ΦST = 0.145 , P > 0.05) 。结合细胞色素b序列分析, 认为两种群可能是从经历了近期瓶颈效应的同一祖先群衍生而来, 并且可将两山系的小熊猫种群作为一个管理单元进行保护和管理。

关键词: 小熊猫, mtDNA, 控制区, 遗传结构, 保护

Abstract: Red panda ( Ailurus fulgens) is an endangered species and survives mainly in Qionglai and Xiangling Mountains in Sichuan Province , China. Twenty-three individuals of the red panda were sampled from these two main mountains ranges with 17 from Xiangling and 6 from Qionglai. Genetic variations within populations and degrees of differentiation between the two populations were analyzed through DNA extracting from hair , PCR amplifying , and sequencing 569 nucleotides of mitochondrial DNA control region. Based on 569 nucleotides , 23 variable sites were determined and 8 haplotypes were defined between the two populations. Both populations exhibited low levels of nucleotide diversity. Slight differentiation about haplotype diversity was identified between both populations , and the Qionglai population had relatively higher degree of diversity than the Xiangling population. It was revealed that geographical partitioning among all haplotypes was incomplete and subdivision level between the two populations was very low due to high gene flow (ΦST = 0.145 , P > 0.05 ; Nm = 2.95) . Combining with cytochrome b data , it is suggested that the two populations might originate from a single ancestral population that has a recent bottleneck effect and might be regarded as one unit for conservation and management.

Key words: Red panda ( Ailurus fulgens), mtDNA, Control region, Genetics structure, Conservation