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塔里木兔种群遗传结构的初步探讨

李增超 夏霖 杨奇森 梁孟元   

  1. 中国科学院动物研究所, 北京
  • 出版日期:2005-10-30 发布日期:2008-07-07

Population Genetic Structure of the Yarkand Hare ( Lepus yarkandensis)

LI Zengchao,XIA Lin,YANGQisen,LIANGMengyuan   

  • Online:2005-10-30 Published:2008-07-07

摘要: 塔里木兔作为中国特有种, 其种群遗传结构直到现在还未知。我们从采自于新疆维吾尔自治区3 个种群的25 号样品中扩增得到529 bp 线粒体DNA 控制区片段, 共发现存在76 个多态性位点。全部样品中定义了19 个单倍型, 每个种群都显示了很高的单倍型多态性。进一步分析表明, 3 个种群存在明显的遗传分化和较低的基因流。通过错配分布和Fu’s 中性检验分析发现, 阿克苏(AKS) , 若羌(RQ) 和疏勒(SL) 种群相对比较稳定。

关键词: 塔里木兔, 线粒体DNA, 控制区, 种群遗传结构

Abstract: The Yarkand hare (Lepus yarkandensis) is one of the endemic species of Lepus from China. The genetic structure of its population remains unknown. We sequenced a 529-bp fragment of the mitochondrial control region (D-loop) from 25 samples of three populations in Xinjiang Uighur Autonomous Region (NW China). Among the sequences, 76 sites were polymorphic. 19 haplotypes were defined; haplotype diversity was 0.964 ±0.00596 in population Aksu (AKS), 0.972 ±0.00409 in population Qarkilik (RQ) and 0.643 ±0.0339 in population Shule (SL). These data indicate a high level of genetic diversity in the Yarkand hare. However, significant genetic subdivision and low gene flow were found among the three populations. From mismatch and Fu's Fs test of neutrality, we found that AKS, RQ, and SL populations are relatively stable.

Key words: Yarkand hare (Lepus yarkandensis), MtDNA, Control region, Population genetic structure