Abstract: Study subjects were 21 giant pandas raised at Chengdu Research Base of Giant Panda Breeding. From 2003.06 to 2005.03, the pandas' diet (consisting of bamboo, fruit, and milk gruel) was supplemented with a specially formulated concentrated biscuit (concentrate) and/or additional vitamin (water scatter lipid vitamins (Aventis AD3 E100-20-20) and B vitamins). Mucous excretion of the pandas was explored for these dietary conditions. Blood levels of B vitamins were examined, and the relationship between the nutritional status of B vitamins and mucous excretion is initially discussed. Results showed that for all three pandas who received supplemental water scatter lipid vitamins and mineral elements, the frequency of mucous excretion was reduced. Of 15 pandas provisioned with the concentrate,14 exhibited reduced mucous excretion. The majority of these pandas did not produce a mucous stool for 7 months. Mucous excretion was also reduced in 6 pandas provisioned with supplementary B vitamin and calcium-magnesium complex. The B1,B6 ,Niacin and Niacinamide concentrations in the serum of 4 pandas who frequently excreted mucous were found to be very low. After the addition of supplementary B vitamins, the concentrations of these vitamins rose and the frequency of mucous excretion decreased. Conclusion: Some of the B vitamins may be related to the frequency of mucous excretion in the captive giant panda. Accordingly, an effective measure to reduce or eliminate mucous excretion in the captive giant panda may be to supply a complete and balanced diet with a careful consideration of micro-nutrients.