关键词: PCR, 小熊猫, 犬细小病毒, 犬冠状病毒

Abstract: We used nested PCR/ RT-PCR method to detect canine distemper virus (CDV),canine parvovirus (CPV),canine adenovirus (CAV),canine coronavirus (CCV),and canine herpesvirus (CHV)in 71 rectal and 61 saliva swabs from captive red pandas without any clinical signs in ten zoos in China to evaluate the threat of captive red pandas by these viruses. Positive PCR products were sequenced and compared with those from the GenBank. We detected three CPV and six CCV positive results from rectal swabs samples,but we found nothing in saliva samples and didn’t detect CDV,CAV
and CHV in any sample. The homologies of CPV and CCV sequences between PCR positive samples and the GenBank are 99% -100% ,respectively. One parvovirus strain was isolated from one of the three parvovirus positive samples. These results showed captive red pandas in China were infected with CPV and CCV,so we should place more emphasis on the prevention of CPV and CCV for better conservation of red pandas. The PCR method can detect trace template of virus and may be a diagnostic method for detecting early viral infection of red pandas.

Key words: Canine parvovirus, Canine coronavirus, PCR, Red panda (Ailurus fulgens)