]*>","")" /> 多位点DNA 指纹技术在保加利亚普通田鼠中的应用探讨

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多位点DNA 指纹技术在保加利亚普通田鼠中的应用探讨

陈炜, Leibenguth Friedrich, 陈宏   

  1. 武汉大学东湖分校生命科学学院,武汉
  • 出版日期:2007-03-17 发布日期:2008-07-07

Studies on applicability of multilocus DNA fingerprinting in Bulgarian common vole (Microtus arvalis)

CHEN Wei, Leibenguth Friedrich, CHEN Hong   

  • Online:2007-03-17 Published:2008-07-07

关键词: DNA 指纹, 普通田鼠, 微卫星探针(GTG), 多态性

Abstract: In the paper, DNA fingerprinting patterns of common vole (Microtus arvalis) were detected with HindIII, HinfI and microsatellite probe (GTG)5. The results showed that legible DNA fingerprints were not produced with HindIII and probe (GTG)5, however HinfI and probe (GTG)5 produced abundantly informative DNA fingerprints. DNA fingerprints were individual-specific and represented higher polymorphism in number, intensity and distribution bands among individuals. The band-sharing rates (BSR) between individuals were determined by the DNA fingerprints. The average band numbersand average band-sharing rates in fragment length region (FLR) of >2.3 kb were 29.625±5.012 and 0.388±0.083 respectively. Correlative fingerprint-data with the population were calculated from average BSR. In this study,a technical basis is established for application of multilocus DNA fingerprinting in common vole.

Key words: Common vole (Microtus arvalis), DNA fingerprinting, Microsatellite probe (GTG)5, Polymorphism