关键词: 鲸类脊椎骨, 细胞色素b 基因, 分子进化树, 种类鉴定, 大村鲸

Abstract: In order to identify an unknown whale vertebra's species,genomic DNA is extracted from the bone powder by proteinase K and phenol /chloroform,and a partial Cyt b gene sequence as long as 565 bp is amplified by PCR (Polymerase Chain Reaction). The blasting result in GenBank shows that the vertebra sample has maximal similarity with Balaenoptera omurai, it is 99%. It indicates that the vertebra is Balaenoptera omurai. The phylogenetic tree based on UPGMA is consistent with the blasting result. Analysis of software MEGA shows that the Cyt b gene sequence of the vertebra sample has 1 -2 nucleotides which are different from the homologous sequence of B. omurai (accession AB201257,AB201256), thus, the sequence is a new haplotype This is the first record of B. omurai which distributes in the South China Sea.

Key words: Balaenoptera omurai, Cytochrome b gene, Phylogenetic tree, Species identification, Whale vertebra