关键词: 大绒鼠, 神经肽Y, 刺鼠相关蛋白, 阿片促黑色素原, 可卡因-, 安他非明转录调节肽, 下丘脑

Abstract: Neuropeptide Y (NPY),agouti related peptide (AgRP),pro-opiomelanocortin (POMC )and cocaine and amphetamine regulated transcript peptide (CART)are four neuropeptides that play essential roles in the regulation of food intake and energy homeostasis in mammals. Partial cDNA sequences of NPY,AgRP,POMC and CART genes were obtained
using the RT-PCR method. The obtained partial sequences of NPY,AgRP,POMC,and CART were 162 bp,187 bp, 240 bp,and 55 bp in length,respectively,which contain open reading frames (ORF)of 114 bp,179 bp,214 bp, 54 bp separately. The protein coding of these sequences include 37 amino acids in NPY;59 amino acids in AgRP;71 amino
acids in POMC;17 amino acids in CART. A search of the BLAST database showed that the deduced sequence of E. miletus NPY,AgRP,POMC and CART were very similar to the NPY,AgRP,POMC and CART of Rattus norvegicus,Mus musculus,and Acomys russatus. In order to make a further determination of the evolutional position of E. miletus,the
NPY,AgRP,POMC and CART genes were used to reconstruct phylogenetic trees by using ML method. The result showed that E. miletus has the closest relationship relative to R. norvegicus and M. musculus. The finding of this comparative study will contribute to further revealing the energy metabolism mechanisms of E. miletus from an evolutionary perspective

Key words: AgRP, CART, Eothenomys miletus, Hypothalamus, NPY, POMC