兽类学报 ›› 2022, Vol. 42 ›› Issue (1): 85-94.DOI: 10.16829/j.slxb.150506

• 研究论文 • 上一篇    下一篇

麦洼牦牛不同生长阶段肝脏差异表达基因分析

傅芳1, 王利1, 字向东2   

  1. 1 西南民族大学, 青藏高原动物遗传资源保护与利用教育部和四川省重点实验室, 成都 610041;
    2 西南民族大学, 动物科学国家民委重点实验室, 成都 610041
  • 收稿日期:2020-11-27 修回日期:2021-06-08 发布日期:2021-06-08
  • 通讯作者: 王利,E-mail:qinxin916@aliyun.com
  • 作者简介:傅芳(1995-),女,硕士研究生,主要从事动物遗传学研究.E-mail:doublefsky0110@163.com
  • 基金资助:
    四川省留学人员科技活动择优资助项目(2019);中央高校基本科研业务费专项资金项目(2021PTJS26)

Differential expression genes analysis of liver in Maiwa yak at different growth stages

FU Fang1, WANG Li1, ZI Xiangdong2   

  1. 1 The Key Laboratory of Qinghai-Tibet Plateau Animal Genetic Resource and Utilization, Ministry of Education and Sichuan Province, Southwest Minzu University, Chengdu 610041, China;
    2 The Key Laboratory of Animal Science, State Ethnic Affairs Commission, Southwest Minzu University, Chengdu 610041, China
  • Received:2020-11-27 Revised:2021-06-08 Published:2021-06-08

摘要: 为探讨牦牛肝脏生长过程中基因的表达特征,采用Illumina高通量测序平台(HiSeqTM2500)对1日龄组(LD)、15月龄组(LM)和5岁龄组(LY)的健康麦洼牦牛肝脏进行转录组测序,并以qRT-PCR验证差异表达基因(differentially expressed genes,DEGs)的表达量。结果显示,LD组、LM组和LY组中分别有325个、85个和84个表达水平高于其他两组的显著差异表达基因(P < 0.05)。在其富集的GO条目和KEGG通路中,分别包含102个、104个和134个显著差异GO条目及19个、13个和19个显著差异KEGG通路(P < 0.05)。其中占比最多的GO条目为氧化还原相关过程、发育过程和代谢过程,占比最多的KEGG通路为磷脂酰肌醇3-激酶-丝氨酸/苏氨酸蛋白激酶B (phosphatidylinositol 3-kinase/protein kinase,PI3K-Akt)信号通路、粘着斑和细胞外基质(extracellular ma-trix,ECM)受体相互作用。qRT-PCR差异验证结果显示,CYP7B1、PGFS2、CYP1A1、UGT2C1-1、UGT2C1L、HSD11B1、CYP2C19、UGT2C1-2基因的表达量变化与转录组测序结果相符。综上表明,本研究为进一步探讨不同生长阶段牦牛肝脏发育提供了有力的实验数据,可为深入理解牦牛肝脏生长发育过程提供参考与借鉴。

关键词: 牦牛, 生长阶段, 肝脏, 转录组

Abstract: The study was conducted to detect the expression pattern of genes in yak liver growth. The transcriptome sequencing of 1-day-old (LD), 15-month-old (LM) and 5-year-old (LY) healthy Maiwa yaks were performed by using Illumina (HiSeqTM2500) high-throughput sequencing platform, and the differentially expressed genes (DEGs) in livers of yaks at different growth stages were verified by qRT-PCR. The results indicated that 325, 85 and 84 significantly DEGs with higher expression levels in LD, LM and LY than the other two groups, respectively (P < 0. 05). The DEGs were significantly enriched in 102, 104 and 134 GO terms and 19, 13 and 19 KEGG pathways, respectively (P < 0. 05). The oxidation-reduction-related process, development process, and metabolic-related process were the largest proportion of GO terms. The PI3K Akt signaling pathway, focal adhesion and ECM receptor interaction were the largest proportion of KEGG pathways. In addition, qRT-PCR results showed that the expressions of CYP7B1, PGFS2, CYP1A1, UGT2C1-1, UGT2C1L, HSD11B1, CYP2C19 and UGT2C1-2 were consistent with those of RNA-Seq. In summary, this study provides powerful experimental data for further exploring the yak liver development at different growth stages and a reference for in-depth understanding of yak liver growth and development process.

Key words: Yak, Growth stages, Liver, Transcriptomics

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