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布氏田鼠对低温的适应性产热

李庆芬, 李宁, 孙儒泳   

  1. 北京师范大学生物系,100875
  • 出版日期:2006-07-29 发布日期:2008-01-05

THE ADAPTIVE THERMOGENESIS 0F BRANDT'S V0LES

LI Qingfen, LI Ning, SUN Ruyong   

  • Online:2006-07-29 Published:2008-01-05

摘要: 将布氏田鼠置l到30天的低温(6℃±1)环境下暴露,其体重与体温没有明显变化;静止代谢率(RMR)、非震颤性产热(NST)及血清中三碘甲腺原氪酸(Ts)含量,随冷暴露时间的延长而增加;肝线粒体状态3呼吸及细胞色素C氧化酶活力亦随冷暴露时间的延长而升高。肝线粒体蛋白质含量经冷暴露30天时有明显升高,表明动物在低温适应中肝线粒体蛋白合成增加与呼吸功能增强是个体RMR提高的细胞学机制之一.布氏田鼠褐色脂肪组织(BAT)的重量及其线粒体蛋白含量在冷暴露1O天后明显增加,其线粒体细胞色素C氧化酶、琥珀酸氧化酶及α-磷酸甘油氧化酶活力均在急性冷暴露环境中明显升高,到30天时可增长2—4倍,表明BAT是动物适应寒冷环境的主要产热器官。

关键词: 布氏田鼠, 冷暴露, 非振颤性产热

Abstract: Compared with brandt's voles living in warm environment(25士2℃ ),the animals
exposed to cold (6士1℃ ) showed some physiological and biochemical changes which might be important in their adaptation to cold.
Cold exposure hadno obvious effects on the body weight and body temperature of the animals.However,resting metabolic rate (RMR ),nonshivering thermogenesis
(NST )and triiodothyronne (T3)concentration in serum increased much more with increasing time.The state-3 respiration and acIivities of cytuchrome C oxidase of liver mitochondria were activated prominently after one day exposure to cold and these activations proceeded with the prolonged cold exposure. The liver motochondrial protein and state—4 respiration also increased after a relatively long period of time(10-20 day)in the cold.
The weights and mituchundrial protein contents of the brown adipose tissue
(BAT)increased after exposure to cold for 1O-20 day.The activities of cytochrome C oxidase,succinate oxidase and α-glycerophosphate oxidase of BAT mitochondria increased markedly after one day exposure to cold and kept the increase with the prolonged cold exposure.The regulation of Ta to RM R and NST was discussed in the article.
It is,therefore,by means of the elevation of RM R and NST of BAT that Brandt's vole can maintain constant body temperature in the cold.

Key words: Brandt's vole(Microtus brandti), Cold exposure, Nonshvering thermogenesis