关键词: 凝血反应, 褐家鼠, 抗药性, 杀鼠灵, 检测

Abstract: To determine the feasibility of a blood clotting response (BCR) method for monitoring the resistance of commensal rodents, Rattus norvegicus were sampled from suburbs of Beijing and discriminated into resistant and susceptible individuals by non-alternative feed test appraised by World Health Organization. When administered with a discrimination dose of Warfarin (12 mg / kg body weight),both resistant and susceptible R. norvegicus in dividuals decreased their percentage clotting activity (PCA) the following day. While PCA of resistant individuals only decreased to 17% of normal level and soon increased to normal level within 2-3 days. In contrast the PCA of susceptible individuals decreased dramatically and no subsequent increase was observed. As a whole population,the distribution pattern of PCA four days of post-treatment R. norvegicus also changes form one peak type of pre-treatment population into double peaks type,one peak stands for resistant individuals and the other for resistance individuals. The peak of the resistant covers PCA range from 0 to 3. 16 and that of susceptible individuals PCA ranges from 17 to 100. In the same time,PCA four days post-treatment also was found to be related to survival of R. norvegicus and can predict survival in subsequent feed test which indicate the consistency of BCR
method and non-alternative feed test in monitoring the resistance of R. norvegicus Therefore, PCA = 17 (INR = 5. 0) should be a threshold in determining responders to discriminate the resistant and susceptible rodent. So BCR might be an applicable method to monitor the resistance against anticoagulant rodenticides of commensal rodents.

Key words: Blood clotting response, Rattus norvegicus, Resistan, Warfarin