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虎血清H5 亚型流感病毒抗体调查

高玉伟, 周明, 刘丹, 王伟, 王立刚, 杨松涛, 朱晓文, 黄耕, 王铁成, 夏咸柱   

  1. 军事医学科学院军事兽医研究所,吉林省人兽共患病预防与控制重点实验室,长春130062
  • 出版日期:2010-11-29 发布日期:2010-03-02

Serologic survey for antibodies to H5 subtype influenza A virus in tigers

GAO Yuwei,ZHOU Ming,LIU Dan,WANG Wei,WANG Ligang,YANG Songtao,ZHU Xiaowen, HUANG Geng,WANG Tiecheng,XIA Xianzhu   

  1. The 11th Institute,Academy of Military Medical Sciences,Changchun 130062,China
  • Online:2010-11-29 Published:2010-03-02

摘要: 为查明我国圈养虎群中H5 亚型流感病毒的流行情况,应用血凝抑制(HI)方法检测了1998 ~ 2009 年间采集于哈尔滨、宜昌、桂林、唐山、上海和郑州等地的309 份虎血清样品的H5 亚型流感病毒抗体效价。结果发现1998 年4 月至2002 年4 月采集的20 份血清样品全部为H5 亚型流感病毒HI 抗体阴性。在2002 年7 月至2003
年6 月采样于上海、唐山、哈尔滨的34 只虎中,有31 只曾出现过高热、抽搐和肺炎症状,其中24 只虎的血清样品H5 亚型流感病毒HI 抗体呈阳性(抗体效价1∶ 10 ~ 1∶ 320),2 只无临床症状虎也为抗体阳性。对2004 年随机采集自哈尔滨的220 份血清样品调查发现抗体阳性率可达25.9% ,其中28 只有临床高热与肺炎病史的虎中有14 只抗体呈阳性(抗体效价1∶ 10 ~1∶ 80),其余无病史的192 只虎中有43 只抗体阳性。2009 年8 月采集的35 份血清中仅有3 份H5 阳性,抗体阳性率下降为8.6% 。上述结果表明H5 亚型流感病毒能够感染虎并对圈养虎的健康构成威胁,而且其公共卫生意义更值得关注。

关键词: 虎, H5 亚型流感病毒, 抗体调查

Abstract:

To explore the prevalence of the H5 subtype influenza A virus in captive tigers in China,the antibodies of 309 tigersserum samples,which were collected from Harbin, Yichang, Guilin, Shanghai, Zhengzhou, and Tangshan between 1998 and 2009,were detected using the hemagglutination inhibition test (HI). All 20 samples collected from April of 1998 to April of 2002 were HI antibody negative. Among 24 of 31 samples collected from July of 2002 to June of 2003 whose clinical signs such as fever and pneumonia were obvious, HI antibody were positive. Surprisingly, the antibody titers of paired sera from two tigers without clinical signs also were HI antibody positive. Of 220 samples randomly collected in 2004 in Harbin,14 of 28 samples collected from tigers with clinical signs were HI antibody positive. In addition, we found that 43 samples collected from tigers without clinical signs also were positive with 110 - 180 antibody titers. In 2009, only 3 out of 43 samples were HI antibody positive. These results indicate that H5 subtype influenza virus have already acquired the ability to infect tigers and pose a threat to captive tigers and public heath.

Key words: H5 subtype influenza A virus, Serologic survey, Tiger