Abstract：The experiment was conducted to establish a Bactrian camel (Camelus bactrianus)fetal ear marginal fibroblast cell line. The Bactrian camel fetal ear marginal tissue was cultured with explants or trypsinization,which then was subcultured and purified through trypsin digestion and differential anchoring velocity. Analysis on cell morphology,cell viability, growth dynamics,immunocytochemistry,karyotype,transfection and expression of green fluorescent protein marker plasmid (GFP),and microbial contamination were carried out. The results showed that the population doubling time (PDT) of the cells was 47 2 h;the majority chromosome number was 74;the immunocytochemistry was positive for vimentin and negative for cytokeratin;an exogenous plasmid could integrate and express in the cells;detection for microbial contamination was negative. As a consequence,we succeeded in establishing the fibroblast cell line of Bactrian camel on cell level which is a significant national germplasm resource,providing ideal experimental material for future studies of the gene bank and somatic cell cloning,etc.