Abstract：The ear tissues of four bovidae species Pantholops hodgsonii,Capra hircus,Ovis aries and Bos taurus were collected and cultured in vitro with DMEM/ F12 media and the cell lines were established.The cell morphologies,growth curves, apoptosis and karyotypes were investigated. The results showed that fibroblast cells from the four different species could be cultured and purified by the combination of differential attachment protocol and digestion elimination methods. The cells had consistent morphology and kept stable vitality. The cell morphologies and growth curves were with not different among the four different species’cells.The population doubling times (PDT)of Pantholops hodgsonii,Capra hircus,Ovis aries and Bos taurus were 26 h,22 h,23 h and 22 h,respectively. The cell apoptosis rates were 5. 5%,2.7% ,8.6% and 1. 5% ,respectively, when assayed by the flow cytometry. The chromosome number of Pantholops hodgsonii,Capra hircus,Ovis aries and Bos taurus are 60,60,54 and 60 respectively. Karyotype analyses showed that Ovis aries have 6 metacentric chromosomes, and those of all of the other species are telocentric type except the bovine X chromosomes are metacentric. These results indicated that the cell growth characteristics of Pantholops hodgsonii had obvious differences when compared to the other Bovidae species. Our research provides a basis to study the molecular mechanisms of Pantholops hodgsonii adaptation to hypoxia.
刘坤 吴侠 魏著英 白春玲 苏广华 李光鹏. 藏羚与几种牛科动物成纤维细胞的特性比较[J]. , 2014, 34(1): 71-79.
LIU Kun,WU Xia,WEI Zhuying,BAI Chunling,SU Guanghua,LI Guangpeng. Comparative of the cytogenetic characteristics of Pantholops hodgsoniiand other three species in Bovidae. , 2014, 34(1): 71-79.