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基于细胞色素氧化酶亚基Ⅰ基因的中缅树鼩地理种群遗传分化

付家豪 王晓 朱万龙 高文荣 王政昆   

  1. 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室,云南师范大学生命科学学院
  • 出版日期:2018-03-30 发布日期:2018-06-19

Genetic variation of different populations in tree shrews(Tupaia belangeri)based on mtDNA cytochrome oxidase subunitⅠgene

FU Jiahao, WANG Xiao, ZHU Wanlong, GAO Wenrong, WANG Zhengkun   

  1. Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of University in Yunnan Province, School of Life Science, Yunnan Normal University
  • Online:2018-03-30 Published:2018-06-19

摘要: 中缅树鼩广泛分布于东南亚地区,在我国主要分布于西南地区及海南岛。本研究以中国10个地理种群共112只中缅树鼩为研究对象, PCR扩增得到1398 bp的细胞色素氧化酶亚基Ⅰ(COⅠ)基因序列,并对其进行分析。 结果显示:112个中缅树鼩COⅠ基因共定义了64个单倍型,其单倍型多样性(Hd)平均值为0.9730,核苷酸多样性(Pi )平均值为0.04494;AMOVA方差分析显示种群间的变异占总变异的93.09%,说明中缅树鼩地理种群变异主要发生在种群间;整体遗传分化固定指数(FST)为0.93091,说明各地理种群中缅树鼩已出现明显的遗传分化;结合中性检验与碱基错配分布图结果表明中缅树鼩在历史进程中未经历过种群扩张现象; 基于单倍型构建的系统进化树与NETWORK网络图显示10个地理种群的中缅树鼩聚为4支:海南种群一支,大新种群一支,片马种群一支,其他种群一支。结果表明:中国不同地理种群的中缅树鼩具有较高的遗传多样性,各地理种群间已经出现了较为明显的遗传分化,地理阻隔作用可能是其分化的主要原因。

关键词: 中缅树鼩, COⅠ基因, 遗传距离

Abstract: Tree shrews (Tupaia belangeri) are widely distributed in southeast Asia, mainly in Hainan Island and southwest regions of China. In the present study, PCR amplification of CO I gene sequence of 1398 BP was used to analyze 10 geographic populations consisting of 112 tree shrews as the research objects,. A total of 64 haplotypes were defined in CO I gene of the 112 samples, the haplotype diversity (Hd) averaged value was 0.9730, and the average value of nucleotide diversity (Pi) was 0.04494; analysis of AMOVA showed that variation among populations accounted for 93.09% of the total variance, suggesting that mutations occurred mainly in populations. The overall genetic differentiation index (FST) fixed to 0.93091, indicating that the populations of tree shrews have obvious genetic differentiation. Combination with neutral inspection and mismatch distribution analysis showed that T. belangeri had not experienced population expansion phenomenon in history . A phylogenetic tree constructed of haplotypes and NETWORK diagram showed that 10 the geographic populations of tree shrews were clustered into 4 branches: Hainan population, Daxin population, Pianma population and other populations. All of the results showed that there was higher genetic diversity of different geographical populations of the China in T. belangeri.  Different populations displayed obvious genetic differentiation and geographical barrier effects may be the main reason for the differentiation.

Key words: COⅠgene, Mean genetic distance, Tupaia belangeri