• 论文 •

### 基于细胞色素氧化酶亚基Ⅰ基因的中缅树鼩地理种群遗传分化

1. 云南省高校西南山地生态系统动植物生态适应进化及保护重点实验室，云南师范大学生命科学学院
• 出版日期:2018-03-30 发布日期:2018-06-19

### Genetic variation of different populations in tree shrews（Tupaia belangeri）based on mtDNA cytochrome oxidase subunitⅠgene

FU Jiahao, WANG Xiao, ZHU Wanlong, GAO Wenrong, WANG Zhengkun

1. Key Laboratory of Ecological Adaptive Evolution and Conservation on Animals-Plants in Southwest Mountain Ecosystem of University in Yunnan Province, School of Life Science, Yunnan Normal University
• Online:2018-03-30 Published:2018-06-19

Abstract: Tree shrews (Tupaia belangeri) are widely distributed in southeast Asia, mainly in Hainan Island and southwest regions of China. In the present study, PCR amplification of CO I gene sequence of 1398 BP was used to analyze 10 geographic populations consisting of 112 tree shrews as the research objects,. A total of 64 haplotypes were defined in CO I gene of the 112 samples, the haplotype diversity (Hd) averaged value was 0.9730, and the average value of nucleotide diversity (Pi) was 0.04494; analysis of AMOVA showed that variation among populations accounted for 93.09% of the total variance, suggesting that mutations occurred mainly in populations. The overall genetic differentiation index (FST) fixed to 0.93091, indicating that the populations of tree shrews have obvious genetic differentiation. Combination with neutral inspection and mismatch distribution analysis showed that T. belangeri had not experienced population expansion phenomenon in history . A phylogenetic tree constructed of haplotypes and NETWORK diagram showed that 10 the geographic populations of tree shrews were clustered into 4 branches: Hainan population, Daxin population, Pianma population and other populations. All of the results showed that there was higher genetic diversity of different geographical populations of the China in T. belangeri.  Different populations displayed obvious genetic differentiation and geographical barrier effects may be the main reason for the differentiation.