• 论文 •

### 小鼠小肠四种消化酶活力的胎后发育模式

1. 沈阳师范大学生命科学学院；广东省生物资源应用研究所，广东省动物保护与资源利用重点实验室，广东省野生动物保护与利用公共实验室
• 出版日期:2018-05-30 发布日期:2018-05-08
• 通讯作者: 刘全生 E–mail: liuqs@giabr.gd.cn
• 基金资助:
国家自然科学基金（31570409）；广东省科学院专项（2016GDASPT-0215,2017GDASCX-0107）

### Various postnatal developmental patterns of four digestive enzyme activities of small intestine in Swiss mouse#br#

LUO Lijuan, WANG Shengnan, YANG Ming, LIU Quansheng

1. College of Life Science, Shenyang Normal University；Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources
• Online:2018-05-30 Published:2018-05-08

Abstract:

We determined the activities of lactase, sucrase, maltase and aminopeptidase-N in small intestine of mice from birth to 27 days of age in order to identify the formation and development model of intestinal digestive enzyme activities in young mice and test the adaptive modulation hypothesis. The lactase specific-mass activity increased initially and then decreased, reachingthe highest levels at 9 days of age in the proximal small intestine and at 12 days of age in the middle and distal small intestine. At 27 days of age, there was weak lactase activity only in the middle small intestine. The sucrase specific-mass activity appeared at 12 days of age, and increased rapidly from 15 days of age, reaching the highest level at 18 days of age in the proximal and distal intestine and at 21 days of age in the middle small intestine, and then declined markedly. The maltase specific-mass activity is weak at birth and remained at this level until 15 days of age. It then increased rapidly and reached the peak at 18 days of age in the proximal and distal intestine and at 21 days of age in the middle intestine, and then decreased. The aminopeptidase-N specific-mass activity of proximal small intestine decline continuously until 27 days of age, but the activity increased continuously from birth to weaning in the middle and distal intestine and then slightly declined. The total lactase activity peaked at 15 days of age and then declined. However, the total activities of sucrase, maltase, and aminopeptidase-N constantly increased through the entire lactation period. All
four enzyme specific-mass activities had remarkable differences among the varied positions of the small intestine. Moreover, the age of mice had significant influence on the distribution of enzyme activity in the different small intestine positions. In sum, the temporal changes of the four kinds of enzymes activities in small intestine could match with the variation of food digestion needs, which partly supported the adaptive modulation hypothesis.